8wm3: Difference between revisions

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-'''Unreleased structure'''
-The entry 8wm3 is ON HOLD
+==Cryo-EM structure of ACE2-SIT1 complex with tiagabine==
+<StructureSection load='8wm3' size='340' side='right'caption='[[8wm3]], [[Resolution|resolution]] 3.34&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8wm3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WM3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.34&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TGI:(3R)-1-[4,4-bis(3-methylthiophen-2-yl)but-3-en-1-yl]piperidine-3-carboxylic+acid'>TGI</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wm3 OCA], [https://pdbe.org/8wm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wm3 RCSB], [https://www.ebi.ac.uk/pdbsum/8wm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wm3 ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/S6A20_HUMAN S6A20_HUMAN] Iminoglycinuria. The disease is caused by variants affecting the gene represented in this entry.  The disease is caused by variants affecting the gene represented in this entry. Haploinsufficiency of SLC6A20 combined with deficiency of the neutral amino acid transporter SLC6A19 or partially inactivating mutations in SLC36A2, is responsible for iminoglycinuria. Additional polymorphisms and mutations in SLC6A18 can contribute to the IG phenotype in some families.
+== Function ==
+[https://www.uniprot.org/uniprot/S6A20_HUMAN S6A20_HUMAN] Mediates the Na(+)- and Cl(-)-dependent uptake of imino acids such as L-proline, N-methyl-L-proline and pipecolate as well as N-methylated amino acids (PubMed:15632147, PubMed:19033659, PubMed:33428810). Also transports glycine, regulates proline and glycine homeostasis in the brain playing a role in the modulation of NMDAR currents (PubMed:33428810).<ref>PMID:15632147</ref> <ref>PMID:19033659</ref> <ref>PMID:33428810</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The pharmacology of amino acid transporters in the SLC6 family is poorly developed compared to that of the neurotransmitter transporters. To identify new inhibitors of the proline transporter SIT1 (SLC6A20), its expression in Xenopus laevis oocytes was optimized. Trafficking of SIT1 was augmented by co-expression of angiotensin-converting enzyme 2 (ACE2) in oocytes but there was no strict requirement for co-expression of ACE2. A pharmacophore-guided screen identified tiagabine as a potent non-competitive inhibitor of SIT1. To understand its binding mode, we determined the cryo-electron microscopy (cryo-EM) structure of ACE2-SIT1 bound with tiagabine. The inhibitor binds close to the orthosteric proline binding site, but due to its size extends into the cytosolic vestibule. This causes the transporter to adopt an inward-open conformation, in which the intracellular gate is blocked. This study provides the first structural insight into inhibition of SIT1 and generates tools for a better understanding of the ACE2-SIT1 complex. These findings may have significance for SARS-CoV-2 binding to its receptor ACE2 in human lung alveolar cells where SIT1 and ACE2 are functionally expressed.
-Authors: Yan, R., Hu, Z., Dai, L.
+Cryo-EM structure of ACE2-SIT1 in complex with tiagabine.,Broer A, Hu Z, Kukulowicz J, Yadav A, Zhang T, Dai L, Bajda M, Yan R, Broer S J Biol Chem. 2024 Aug 17;300(9):107687. doi: 10.1016/j.jbc.2024.107687. PMID:39159813<ref>PMID:39159813</ref>
-Description: Cryo-EM structure of ACE2-SIT1 complex with Tiagabine
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Hu, Z]]
+<div class="pdbe-citations 8wm3" style="background-color:#fffaf0;"></div>
-[[Category: Dai, L]]
+== References ==
-[[Category: Yan, R]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Dai L]]
+[[Category: Hu Z]]
+[[Category: Yan R]]
+[[Category: Zhang T]]