1oww: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1oww.gif|left|200px]]<br />
-<applet load="1oww" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1oww" />
-'''Solution structure of the first type III module of human fibronectin determined by 1H, 15N NMR spectroscopy'''<br />
-==Overview==
+==Solution structure of the first type III module of human fibronectin determined by 1H, 15N NMR spectroscopy==
-Fibronectin (FN) forms fibrillar networks coupling cells to the, extracellular matrix. The formation of FN fibrils, fibrillogenesis, is a, tightly regulated process involving the exposure of cryptic binding sites, in individual FN type III (FN-III) repeats presumably exposed by, mechanical tension. The FN-III1 module has been previously proposed to, contain such cryptic sites that promote the assembly of extracellular, matrix FN fibrils. We have combined NMR and steered molecular dynamics, simulations to study the structure and mechanical unfolding pathway of, FN-III1. This study finds that FN-III1 consists of a beta-sandwich, structure that unfolds to a mechanically stable intermediate about four, times the length of the native folded state. Considering previous, experimental findings, our studies provide a structural model by which, mechanical stretching of FN-III1 may induce fibrillogenesis through this, partially unfolded intermediate.
+<StructureSection load='1oww' size='340' side='right'caption='[[1oww]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1oww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OWW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oww OCA], [https://pdbe.org/1oww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oww RCSB], [https://www.ebi.ac.uk/pdbsum/1oww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oww ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/FINC_HUMAN FINC_HUMAN] Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2) [MIM:[https://omim.org/entry/601894 601894]; also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life.<ref>PMID:18268355</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/FINC_HUMAN FINC_HUMAN] Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.<ref>PMID:8114919</ref> <ref>PMID:11209058</ref> <ref>PMID:15665290</ref> <ref>PMID:19379667</ref>   Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.<ref>PMID:8114919</ref> <ref>PMID:11209058</ref> <ref>PMID:15665290</ref> <ref>PMID:19379667</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/1oww_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oww ConSurf].
+<div style="clear:both"></div>
-==Disease==
+==See Also==
-Known diseases associated with this structure: Ehlers-Danlos syndrome, type X, 225310 (1) OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=135600 135600]]
+*[[Fibronectin 3D structures|Fibronectin 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-OWW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OWW OCA].
+__TOC__
+</StructureSection>
-==Reference==
-Structure and functional significance of mechanically unfolded fibronectin type III1 intermediates., Gao M, Craig D, Lequin O, Campbell ID, Vogel V, Schulten K, Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14784-9. Epub 2003 Dec 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14657397 14657397]
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Campbell, I.D.]]
+[[Category: Campbell ID]]
-[[Category: Craig, D.]]
+[[Category: Craig D]]
-[[Category: Gao, M.]]
+[[Category: Gao M]]
-[[Category: Lequin, O.]]
+[[Category: Lequin O]]
-[[Category: Schulten, K.]]
+[[Category: Schulten K]]
-[[Category: Vogel, V.]]
+[[Category: Vogel V]]
-[[Category: fibronectin type iii module]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:37:49 2007''