6wp8: Difference between revisions

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<StructureSection load='6wp8' size='340' side='right'caption='[[6wp8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='6wp8' size='340' side='right'caption='[[6wp8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6wp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mastigocladopsis_repens Mastigocladopsis repens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WP8 FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WP8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp8 OCA], [https://pdbe.org/6wp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wp8 RCSB], [https://www.ebi.ac.uk/pdbsum/6wp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp8 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp8 OCA], [https://pdbe.org/6wp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wp8 RCSB], [https://www.ebi.ac.uk/pdbsum/6wp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp8 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microbial rhodopsins are versatile and ubiquitous retinal-binding proteins which function as light-driven ion pumps, light-gated ion channels and photosensors, with potential utility as optogenetic tools for altering membrane potential in target cells. Insights from crystal structures have been central for understanding proton, sodium, and chloride transport mechanisms of microbial rhodopsins. Two of three known groups of anion pumps, the archaeal halorhodopsins (HRs) and bacterial chloride-pumping rhodopsins (ClRs), have been structurally characterized. Here we report the structure of a representative of a recently discovered third group consisting of cyanobacterial chloride and sulfate ion-pumping rhodopsins, the Mastigocladopsis repens rhodopsin (MastR). Chloride-pumping MastR contains in its ion transport pathway a unique Thr-Ser-Asp (TSD) motif, which is involved in binding of a chloride ion. The structure reveals that the chloride-binding mode is more similar to HRs than ClRs, but the overall structure most closely resembles bacteriorhodopsin (BR), an archaeal proton pump. The MastR structure shows a trimer arrangement reminiscent of BR-like proton pumps and shows features at the extracellular side more similar to BR than the other chloride pumps. We further solved the structure of the MastR-T74D mutant which contains a single amino acid replacement in the TSD motif. We provide insights into why this point mutation can convert the MastR chloride pump into a proton pump, but cannot in HRs. Our study points at the importance of precise coordination and exact location of the water molecule in the active center of proton pumps, which serves as a bridge for the key proton transfer.
The crystal structures of a chloride-pumping microbial rhodopsin and its proton-pumping mutant illuminate proton transfer determinants.,Besaw JE, Ou WL, Morizumi T, Eger BT, Sanchez Vasquez JD, Chu JHY, Harris A, Brown LS, Miller RJD, Ernst OP J Biol Chem. 2020 Jul 23. pii: RA120.014118. doi: 10.1074/jbc.RA120.014118. PMID:32703899<ref>PMID:32703899</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6wp8" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mastigocladopsis repens]]
[[Category: Besaw JE]]
[[Category: Besaw JE]]
[[Category: Ernst OP]]
[[Category: Ernst OP]]
[[Category: Morizumi T]]
[[Category: Morizumi T]]
[[Category: Ou W]]
[[Category: Ou W]]

Latest revision as of 12:16, 9 October 2024

Proton-pumping mutant of Mastigocladopsis repens rhodopsin chloride pumpProton-pumping mutant of Mastigocladopsis repens rhodopsin chloride pump

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

6wp8, resolution 2.50Å

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