1nb2: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1nb2.jpg|left|200px]]
-<!--
+==Crystal Structure of Nucleoside Diphosphate Kinase from Bacillus Halodenitrificans==
-The line below this paragraph, containing "STRUCTURE_1nb2", creates the "Structure Box" on the page.
+<StructureSection load='1nb2' size='340' side='right'caption='[[1nb2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1nb2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Virgibacillus_halodenitrificans Virgibacillus halodenitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NB2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nb2 OCA], [https://pdbe.org/1nb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nb2 RCSB], [https://www.ebi.ac.uk/pdbsum/1nb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nb2 ProSAT]</span></td></tr>
-{{STRUCTURE_1nb2|  PDB=1nb2  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q7SIA9_VIRHA Q7SIA9_VIRHA] Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.[HAMAP-Rule:MF_00451]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nb/1nb2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nb2 ConSurf].
+<div style="clear:both"></div>
-'''Crystal Structure of Nucleoside Diphosphate Kinase from Bacillus Halodenitrificans'''
+==See Also==
+*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-We found that when grown under anaerobic conditions the moderate halophile, gram-positive bacterium Bacillus halodenitrificans (ATCC 49067) synthesizes large amounts of a polypeptide complex that contains a heme center capable of reversibly bind nitric oxide. This complex, when exposed to air, dissociates and reassociates into two active components, a Mn-containing superoxide dismutase (SOD) and a nucleoside diphosphate kinase (BhNDK). The crystal structure of this latter enzyme has been determined at 2.2A resolution using molecular replacement method, based on the crystal structure of Drosophila melanogaster NDK. The model contains 149 residues of a total 150 residues and 34 water molecules. BhNDK consists of a four-stranded antiparallel beta-sheet, whose surfaces are partially covered by six alpha-helices, and its overall and active site structures are similar to those of homologous enzymes. However, the hexameric packing of BhNDK shows that this enzyme is different from both eukaryotic and gram-negative bacteria. The need for the bacterium to presynthesize both SOD and NDK precursors which are activated during the anaerobic-aerobic transition is discussed.
+[[Category: Large Structures]]
-==About this Structure==
-NB2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Virgibacillus_halodenitrificans Virgibacillus halodenitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NB2 OCA].
-==Reference==
-Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans: coexpression of its activity with a Mn-superoxide dismutase., Chen CJ, Liu MY, Chang T, Chang WC, Wang BC, Le Gall J, J Struct Biol. 2003 May;142(2):247-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12713952 12713952]
-[[Category: Nucleoside-diphosphate kinase]]
-[[Category: Single protein]]
 [[Category: Virgibacillus halodenitrificans]]
-[[Category: Chang, T.]]
+[[Category: Chang T]]
-[[Category: Chang, W C.]]
+[[Category: Chang W-C]]
-[[Category: Chen, C J.]]
+[[Category: Chen C-J]]
-[[Category: Gall, J Le.]]
+[[Category: Le Gall J]]
-[[Category: Liu, M Y.]]
+[[Category: Liu M-Y]]
-[[Category: Wang, B C.]]
+[[Category: Wang B-C]]
-[[Category: Bacillus halodenitrifian]]
-[[Category: Nucleoside diphosphate kinase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:19:00 2008''