8i3d: Difference between revisions

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== Function ==
== Function ==
[https://www.uniprot.org/uniprot/AB25G_ARATH AB25G_ARATH] High affinity abscisic acid (ABA) transporter that mediates the export of ABA, with a preference for (+)-ABA, through the plasma membrane, especially in vascular tissues (e.g. phloem companion cells), and is involved in the intercellular ABA signaling pathway (PubMed:20133881, PubMed:20935463, PubMed:24521878, PubMed:26517905). Together with ABCG31, export ABA from the endosperm to deliver it to the embryo via ABCG30 and ABCG40-mediated import to suppress radicle extension and subsequent embryonic growth (PubMed:26334616).<ref>PMID:20133881</ref> <ref>PMID:24521878</ref> <ref>PMID:26334616</ref> <ref>PMID:20935463</ref> <ref>PMID:26517905</ref>  
[https://www.uniprot.org/uniprot/AB25G_ARATH AB25G_ARATH] High affinity abscisic acid (ABA) transporter that mediates the export of ABA, with a preference for (+)-ABA, through the plasma membrane, especially in vascular tissues (e.g. phloem companion cells), and is involved in the intercellular ABA signaling pathway (PubMed:20133881, PubMed:20935463, PubMed:24521878, PubMed:26517905). Together with ABCG31, export ABA from the endosperm to deliver it to the embryo via ABCG30 and ABCG40-mediated import to suppress radicle extension and subsequent embryonic growth (PubMed:26334616).<ref>PMID:20133881</ref> <ref>PMID:24521878</ref> <ref>PMID:26334616</ref> <ref>PMID:20935463</ref> <ref>PMID:26517905</ref>  
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== Publication Abstract from PubMed ==
Abscisic acid (ABA) is one of the plant hormones that regulate various physiological processes, including stomatal closure, seed germination and development. ABA is synthesized mainly in vascular tissues and transported to distal sites to exert its physiological functions. Many ABA transporters have been identified, however, the molecular mechanism of ABA transport remains elusive. Here we report the cryogenic electron microscopy structure of the Arabidopsis thaliana adenosine triphosphate-binding cassette G subfamily ABA exporter ABCG25 (AtABCG25) in inward-facing apo conformation, ABA-bound pre-translocation conformation and outward-facing occluded conformation. Structural and biochemical analyses reveal that the ABA bound with ABCG25 adopts a similar configuration as that in ABA receptors and that the ABA-specific binding is dictated by residues from transmembrane helices TM1, TM2 and TM5a of each protomer at the transmembrane domain interface. Comparison of different conformational structures reveals conformational changes, especially those of transmembrane helices and residues constituting the substrate translocation pathway during the cross-membrane transport process. Based on the structural data, a 'gate-flipper' translocation model of ABCG25-mediated ABA cross-membrane transport is proposed. Our structural data on AtABCG25 provide new clues to the physiological study of ABA and shed light on its potential applications in plants and agriculture.
Cryo-EM structure and molecular mechanism of abscisic acid transporter ABCG25.,Huang X, Zhang X, An N, Zhang M, Ma M, Yang Y, Jing L, Wang Y, Chen Z, Zhang P Nat Plants. 2023 Sep 4. doi: 10.1038/s41477-023-01509-7. PMID:37666961<ref>PMID:37666961</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
== References ==
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Latest revision as of 14:18, 14 March 2024

Cryo-EM structure of abscisic acid transporter AtABCG25Cryo-EM structure of abscisic acid transporter AtABCG25

Structural highlights

8i3d is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.81Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AB25G_ARATH High affinity abscisic acid (ABA) transporter that mediates the export of ABA, with a preference for (+)-ABA, through the plasma membrane, especially in vascular tissues (e.g. phloem companion cells), and is involved in the intercellular ABA signaling pathway (PubMed:20133881, PubMed:20935463, PubMed:24521878, PubMed:26517905). Together with ABCG31, export ABA from the endosperm to deliver it to the embryo via ABCG30 and ABCG40-mediated import to suppress radicle extension and subsequent embryonic growth (PubMed:26334616).[1] [2] [3] [4] [5]

References

  1. ↑ Kuromori T, Miyaji T, Yabuuchi H, Shimizu H, Sugimoto E, Kamiya A, Moriyama Y, Shinozaki K. ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2361-6. PMID:20133881 doi:10.1073/pnas.0912516107
  2. ↑ Kuromori T, Sugimoto E, Shinozaki K. Intertissue signal transfer of abscisic acid from vascular cells to guard cells. Plant Physiol. 2014 Apr;164(4):1587-92. PMID:24521878 doi:10.1104/pp.114.235556
  3. ↑ Kang J, Yim S, Choi H, Kim A, Lee KP, Lopez-Molina L, Martinoia E, Lee Y. Abscisic acid transporters cooperate to control seed germination. Nat Commun. 2015 Sep 3;6:8113. PMID:26334616 doi:10.1038/ncomms9113
  4. ↑ Kuromori T, Shinozaki K. ABA transport factors found in Arabidopsis ABC transporters. Plant Signal Behav. 2010 Sep;5(9):1124-6. PMID:20935463 doi:10.4161/psb.5.9.12566
  5. ↑ Borghi L, Kang J, Ko D, Lee Y, Martinoia E. The role of ABCG-type ABC transporters in phytohormone transport. Biochem Soc Trans. 2015 Oct;43(5):924-30. PMID:26517905 doi:10.1042/BST20150106

8i3d, resolution 2.81Å

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