8u5d: Difference between revisions

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-'''Unreleased structure'''
-The entry 8u5d is ON HOLD
+==Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Space Group P 41 21 2==
+<StructureSection load='8u5d' size='340' side='right'caption='[[8u5d]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8u5d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U5D FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u5d OCA], [https://pdbe.org/8u5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u5d RCSB], [https://www.ebi.ac.uk/pdbsum/8u5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u5d ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF] This enterotoxin is responsible for many cases of a mild type of food poisoning.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Clostridium perfringens enterotoxin (CpE) is a beta-pore forming toxin that disrupts gastrointestinal homeostasis in mammals by binding membrane protein receptors called claudins. Although structures of CpE fragments bound to claudins have been determined, the mechanisms that trigger CpE activation and oligomerization that lead to the formation of cytotoxic beta-pores remain undetermined. Proteolysis of CpE in the gut by trypsin has been shown to play a role in this and subsequent cytotoxicity processes. Here, we report solution structures of full-length and trypsinized CpE using small-angle X-ray scattering (SAXS) and crystal structures of trypsinized CpE and its C-terminal claudin-binding domain (cCpE) using X-ray crystallography. Mass spectrometry and SAXS uncover that removal of the CpE N-terminus by trypsin alters the CpE structure to expose areas that are normally unexposed. Crystal structures of trypsinized CpE and cCpE reveal unique dimer interfaces that could serve as oligomerization sites. Moreover, comparisons of these structures to existing ones predict the functional implications of oligomerization in the contexts of cell receptor binding and beta-pore formation. This study sheds light on trypsin's role in altering CpE structure to activate its function via inducing oligomerization on its path toward cytotoxic beta-pore formation. Its findings can incite new approaches to inhibit CpE-based cytotoxicity with oligomer-disrupting therapeutics.
-Authors: Kapoor, S., Vecchio, A.J.
+Structural Basis of Clostridium perfringens Enterotoxin Activation and Oligomerization by Trypsin.,Ogbu CP, Kapoor S, Vecchio AJ Toxins (Basel). 2023 Oct 31;15(11):637. doi: 10.3390/toxins15110637. PMID:37999500<ref>PMID:37999500</ref>
-Description: Crystal Structure of C-terminal domain of Clostridium perfringens Enterotoxin in Space Group P 41 21 2
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Kapoor, S]]
+<div class="pdbe-citations 8u5d" style="background-color:#fffaf0;"></div>
-[[Category: Vecchio, A.J]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Clostridium perfringens]]
+[[Category: Large Structures]]
+[[Category: Kapoor S]]
+[[Category: Vecchio AJ]]