1mcv: Difference between revisions

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[[Image:1mcv.jpg|left|200px]]


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==Crystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic Elastase==
The line below this paragraph, containing "STRUCTURE_1mcv", creates the "Structure Box" on the page.
<StructureSection load='1mcv' size='340' side='right'caption='[[1mcv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1mcv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MCV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1mcv|  PDB=1mcv |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mcv OCA], [https://pdbe.org/1mcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mcv RCSB], [https://www.ebi.ac.uk/pdbsum/1mcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mcv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG] Acts upon elastin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/1mcv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mcv ConSurf].
<div style="clear:both"></div>


'''Crystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic Elastase'''
==See Also==
 
*[[Elastase 3D structures|Elastase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The crystal structure of porcine pancreatic elastase in complex with a hybrid squash inhibitor (HEI-TOE I; 28 amino acids) has been determined to a resolution of 1.8 A. To construct the hybrid inhibitor, the trypsin-binding loop of the squash inhibitor from Ecballium elaterium was substituted by the sequence of a peptide that was derived from the third domain of the turkey ovomucoid inhibitor and was optimized to inhibit porcine pancreatic elastase. This modification of the squash inhibitor changed its specificity for trypsin to a specificity for porcine pancreatic elastase. Specific interactions of this hybrid inhibitor with porcine pancreatic elastase and the differences from the interactions of the ovomucoid inhibitor with human leukocyte elastase are discussed. The binding loop of the inhibitor adopts a 'canonical' conformation and the scissile bond Leu-Glu remains intact.
[[Category: Large Structures]]
 
==About this Structure==
1MCV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MCV OCA].
 
==Reference==
Structure of a hybrid squash inhibitor in complex with porcine pancreatic elastase at 1.8 A resolution., Ay J, Hilpert K, Krauss N, Schneider-Mergener J, Hohne W, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):247-54. Epub 2003, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12554935 12554935]
[[Category: Pancreatic elastase]]
[[Category: Single protein]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Ay, J.]]
[[Category: Ay J]]
[[Category: Hilpert, K.]]
[[Category: Hilpert K]]
[[Category: Hoehne, W.]]
[[Category: Hoehne W]]
[[Category: Krauss, N.]]
[[Category: Krauss N]]
[[Category: Schneider-Mergener, J.]]
[[Category: Schneider-Mergener J]]
[[Category: Elastase-inhibitor complex]]
[[Category: Hybrid squash inhibitor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:53:46 2008''

Latest revision as of 11:34, 10 April 2024

Crystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic ElastaseCrystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic Elastase

Structural highlights

1mcv is a 2 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CELA1_PIG Acts upon elastin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1mcv, resolution 1.80Å

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