1lv2: Difference between revisions

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New page: left|200px<br /> <applet load="1lv2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lv2, resolution 2.7Å" /> '''Hepatocyte Nuclear F...
 
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[[Image:1lv2.gif|left|200px]]<br />
<applet load="1lv2" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lv2, resolution 2.7&Aring;" />
'''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''<br />


==Overview==
==Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids==
The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain, (LBD) revealed the presence of a fatty acid within the pocket, with the, AF2 helix in a conformation characteristic of a transcriptionally active, nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts, from purified HNF4alpha and HNF4gamma LBDs identified mixtures of, saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4, LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes, show high constitutive activity in transient transfection assays, which, was reduced by mutations designed to interfere with fatty acid binding., The endogenous fatty acids did not readily exchange with radiolabeled, palmitic acid, and all attempts to displace them without denaturing the, protein failed. Our results suggest that the HNF4s may be transcription, factors that are constitutively bound to fatty acids.
<StructureSection load='1lv2' size='340' side='right'caption='[[1lv2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1lv2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LV2 FirstGlance]. <br>
1LV2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PLM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LV2 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lv2 OCA], [https://pdbe.org/1lv2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lv2 RCSB], [https://www.ebi.ac.uk/pdbsum/1lv2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lv2 ProSAT]</span></td></tr>
Hepatocyte nuclear factor 4 is a transcription factor that constitutively binds fatty acids., Wisely GB, Miller AB, Davis RG, Thornquest AD Jr, Johnson R, Spitzer T, Sefler A, Shearer B, Moore JT, Miller AB, Willson TM, Williams SP, Structure. 2002 Sep;10(9):1225-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12220494 12220494]
</table>
== Function ==
[https://www.uniprot.org/uniprot/HNF4G_HUMAN HNF4G_HUMAN] Transcription factor. Has a lower transcription activation potential than HNF4-alpha.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/1lv2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lv2 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Davis, R.G.]]
[[Category: Davis RG]]
[[Category: Johnson, R.]]
[[Category: Johnson R]]
[[Category: Miller, A.B.]]
[[Category: Miller AB]]
[[Category: Moore, J.T.]]
[[Category: Moore JT]]
[[Category: Sefler, A.]]
[[Category: Sefler A]]
[[Category: Shearer, B.]]
[[Category: Shearer B]]
[[Category: Spitzer, T.]]
[[Category: Spitzer T]]
[[Category: Williams, S.P.]]
[[Category: Williams SP]]
[[Category: Willson, T.M.]]
[[Category: Willson TM]]
[[Category: Wisely, B.]]
[[Category: Wisely B]]
[[Category: PLM]]
[[Category: diabetes]]
[[Category: fatty acids]]
[[Category: hnf4]]
[[Category: mody]]
[[Category: nuclear receptor]]
[[Category: transcription factor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:04:24 2007''

Latest revision as of 10:36, 14 February 2024

Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty AcidsHepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids

Structural highlights

1lv2 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HNF4G_HUMAN Transcription factor. Has a lower transcription activation potential than HNF4-alpha.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1lv2, resolution 2.70Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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