1ls6: Difference between revisions

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OCA

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-[[Image:1ls6.gif|left|200px]]<br />
-<applet load="1ls6" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ls6, resolution 1.90&Aring;" />
-'''Human SULT1A1 complexed with PAP and p-Nitrophenol'''<br />
-==Overview==
+==Human SULT1A1 complexed with PAP and p-Nitrophenol==
-Sulfonation catalyzed by sulfotransferase enzymes plays an important role, in chemical defense mechanisms against various xenobiotics but also, bioactivates carcinogens. A major human sulfotransferase, SULT1A1, metabolizes and/or bioactivates many endogenous compounds and is, implicated in a range of cancers because of its ability to modify diverse, promutagen and procarcinogen xenobiotics. The crystal structure of human, SULT1A1 reported here is the first sulfotransferase structure complexed, with a xenobiotic substrate. An unexpected finding is that the enzyme, accommodates not one but two molecules of the xenobiotic model substrate, p-nitrophenol in the active site. This result is supported by kinetic data, for SULT1A1 that show substrate inhibition for this small xenobiotic. The, extended active site of SULT1A1 is consistent with binding of, diiodothyronine but cannot easily accommodate beta-estradiol, although, both are known substrates. This observation, together with evidence for a, disorder-order transition in SULT1A1, suggests that the active site is, flexible and can adapt its architecture to accept diverse hydrophobic, substrates with varying sizes, shapes and flexibility. Thus the crystal, structure of SULT1A1 provides the molecular basis for substrate inhibition, and reveals the first clues as to how the enzyme sulfonates a wide variety, of lipophilic compounds.
+<StructureSection load='1ls6' size='340' side='right'caption='[[1ls6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ls6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LS6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ls6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ls6 OCA], [https://pdbe.org/1ls6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ls6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ls6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ls6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1ls6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ls6 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-LS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with A3P and NPO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LS6 OCA].
+*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structure of a human carcinogen-converting enzyme, SULT1A1. Structural and kinetic implications of substrate inhibition., Gamage NU, Duggleby RG, Barnett AC, Tresillian M, Latham CF, Liyou NE, McManus ME, Martin JL, J Biol Chem. 2003 Feb 28;278(9):7655-62. Epub 2002 Dec 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12471039 12471039]
+__TOC__
-[[Category: Aryl sulfotransferase]]
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Barnett, A.C.]]
+[[Category: Barnett AC]]
-[[Category: Gamage, N.U.]]
+[[Category: Gamage NU]]
-[[Category: Latham, C.F.]]
+[[Category: Latham CF]]
-[[Category: Liyou, N.E.]]
+[[Category: Liyou NE]]
-[[Category: Martin, J.L.]]
+[[Category: Martin JL]]
-[[Category: McManus, M.E.]]
+[[Category: McManus ME]]
-[[Category: Tresillian, M.]]
+[[Category: Tresillian M]]
-[[Category: A3P]]
-[[Category: NPO]]
-[[Category: p-nitrophenol]]
-[[Category: pap]]
-[[Category: positive cooperativity]]
-[[Category: sult 1a1]]
-[[Category: two substrate binding sites]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:03:51 2007''