8pyh: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8pyh is ON HOLD  until Paper Publication
+==Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Crinalium epipsammum PCC 9333==
+<StructureSection load='8pyh' size='340' side='right'caption='[[8pyh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8pyh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crinalium_epipsammum_PCC_9333 Crinalium epipsammum PCC 9333]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PYH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pyh OCA], [https://pdbe.org/8pyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pyh RCSB], [https://www.ebi.ac.uk/pdbsum/8pyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pyh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/K9VSN3_9CYAN K9VSN3_9CYAN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Orange Carotenoid Protein (OCP) is a unique photoreceptor crucial for cyanobacterial photoprotection. Best studied Synechocystis sp. PCC 6803 OCP belongs to the large OCP1 family. Downregulated by the Fluorescence Recovery Protein (FRP) in low-light, high-light-activated OCP1 binds to the phycobilisomes and performs non-photochemical quenching. Recently discovered families OCP2 and OCP3 remain structurally and functionally underexplored, and no systematic comparative studies have ever been conducted. Here we present two first crystal structures of OCP2 from morphoecophysiologically different cyanobacteria and provide their comprehensive structural, spectroscopic and functional comparison with OCP1, the recently described OCP3 and all-OCP ancestor. Structures enable correlation of spectroscopic signatures with the effective number of hydrogen and discovered here chalcogen bonds anchoring the ketocarotenoid in OCP, as well as with the rotation of the echinenone's beta-ionone ring in the CTD. Structural data also helped rationalize the observed differences in OCP/FRP and OCP/phycobilisome functional interactions. These data are expected to foster OCP research and applications in optogenetics, targeted carotenoid delivery and cyanobacterial biomass engineering.
-Authors: Boyko, K.M., Varfolomeeva, L.A., Slonimskiy, Y.B., Maksimov, E.G., Popov, V.O., Sluchanko, N.N.
+Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families.,Sluchanko NN, Maksimov EG, Slonimskiy YB, Varfolomeeva LA, Bukhanko AY, Egorkin NA, Tsoraev GV, Khrenova MG, Ge B, Qin S, Boyko KM, Popov VO Int J Biol Macromol. 2024 Jan;254(Pt 2):127874. doi: , 10.1016/j.ijbiomac.2023.127874. Epub 2023 Nov 7. PMID:37939760<ref>PMID:37939760</ref>
-Description: Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Crinalium epipsammum PCC 9333
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Slonimskiy, Y.B]]
+<div class="pdbe-citations 8pyh" style="background-color:#fffaf0;"></div>
-[[Category: Sluchanko, N.N]]
+== References ==
-[[Category: Varfolomeeva, L.A]]
+<references/>
-[[Category: Popov, V.O]]
+__TOC__
-[[Category: Maksimov, E.G]]
+</StructureSection>
-[[Category: Boyko, K.M]]
+[[Category: Crinalium epipsammum PCC 9333]]
+[[Category: Large Structures]]
+[[Category: Boyko KM]]
+[[Category: Maksimov EG]]
+[[Category: Popov VO]]
+[[Category: Slonimskiy YB]]
+[[Category: Sluchanko NN]]
+[[Category: Varfolomeeva LA]]