8thm: Difference between revisions
New page: '''Unreleased structure''' The entry 8thm is ON HOLD Authors: Pulsford, S.B., Jackson, C.J. Description: Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001 [[Category: ... |
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==Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001== | |||
<StructureSection load='8thm' size='340' side='right'caption='[[8thm]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8thm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanobium_sp._PCC_7001 Cyanobium sp. PCC 7001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8THM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8THM FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8thm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8thm OCA], [https://pdbe.org/8thm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8thm RCSB], [https://www.ebi.ac.uk/pdbsum/8thm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8thm ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B5ILN4_9CYAN B5ILN4_9CYAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cyanobacterial CO(2) concentrating mechanisms (CCMs) sequester a globally consequential proportion of carbon into the biosphere. Proteinaceous microcompartments, called carboxysomes, play a critical role in CCM function, housing two enzymes to enhance CO(2) fixation: carbonic anhydrase (CA) and Rubisco. Despite its importance, our current understanding of the carboxysomal CAs found in alpha-cyanobacteria, CsoSCA, remains limited, particularly regarding the regulation of its activity. Here, we present a structural and biochemical study of CsoSCA from the cyanobacterium Cyanobium sp. PCC7001. Our results show that the Cyanobium CsoSCA is allosterically activated by the Rubisco substrate ribulose-1,5-bisphosphate and forms a hexameric trimer of dimers. Comprehensive phylogenetic and mutational analyses are consistent with this regulation appearing exclusively in cyanobacterial alpha-carboxysome CAs. These findings clarify the biologically relevant oligomeric state of alpha-carboxysomal CAs and advance our understanding of the regulation of photosynthesis in this globally dominant lineage. | |||
Cyanobacterial alpha-carboxysome carbonic anhydrase is allosterically regulated by the Rubisco substrate RuBP.,Pulsford SB, Outram MA, Forster B, Rhodes T, Williams SJ, Badger MR, Price GD, Jackson CJ, Long BM Sci Adv. 2024 May 10;10(19):eadk7283. doi: 10.1126/sciadv.adk7283. Epub 2024 May , 10. PMID:38728392<ref>PMID:38728392</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Jackson | <div class="pdbe-citations 8thm" style="background-color:#fffaf0;"></div> | ||
[[Category: Pulsford | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cyanobium sp. PCC 7001]] | |||
[[Category: Large Structures]] | |||
[[Category: Jackson CJ]] | |||
[[Category: Pulsford SB]] | |||
Latest revision as of 08:29, 28 August 2024
Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001
Structural highlights
FunctionPublication Abstract from PubMedCyanobacterial CO(2) concentrating mechanisms (CCMs) sequester a globally consequential proportion of carbon into the biosphere. Proteinaceous microcompartments, called carboxysomes, play a critical role in CCM function, housing two enzymes to enhance CO(2) fixation: carbonic anhydrase (CA) and Rubisco. Despite its importance, our current understanding of the carboxysomal CAs found in alpha-cyanobacteria, CsoSCA, remains limited, particularly regarding the regulation of its activity. Here, we present a structural and biochemical study of CsoSCA from the cyanobacterium Cyanobium sp. PCC7001. Our results show that the Cyanobium CsoSCA is allosterically activated by the Rubisco substrate ribulose-1,5-bisphosphate and forms a hexameric trimer of dimers. Comprehensive phylogenetic and mutational analyses are consistent with this regulation appearing exclusively in cyanobacterial alpha-carboxysome CAs. These findings clarify the biologically relevant oligomeric state of alpha-carboxysomal CAs and advance our understanding of the regulation of photosynthesis in this globally dominant lineage. Cyanobacterial alpha-carboxysome carbonic anhydrase is allosterically regulated by the Rubisco substrate RuBP.,Pulsford SB, Outram MA, Forster B, Rhodes T, Williams SJ, Badger MR, Price GD, Jackson CJ, Long BM Sci Adv. 2024 May 10;10(19):eadk7283. doi: 10.1126/sciadv.adk7283. Epub 2024 May , 10. PMID:38728392[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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