8te8: Difference between revisions

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'''Unreleased structure'''


The entry 8te8 is ON HOLD  until Paper Publication
==Crystal Structure of Pyridoxal Reductase (PDXI)==
 
<StructureSection load='8te8' size='340' side='right'caption='[[8te8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
Authors: Donkor, A.K., Safo, M.K., Musayev, F.N.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[8te8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TE8 FirstGlance]. <br>
Description: Crystal Structure of Pyridoxal Reductase (PDXI)
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8te8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8te8 OCA], [https://pdbe.org/8te8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8te8 RCSB], [https://www.ebi.ac.uk/pdbsum/8te8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8te8 ProSAT]</span></td></tr>
[[Category: Musayev, F.N]]
</table>
[[Category: Donkor, A.K]]
== Function ==
[[Category: Safo, M.K]]
[https://www.uniprot.org/uniprot/PDXI_ECOLI PDXI_ECOLI] Catalyzes the NAD(P)H-dependent reduction of pyridoxal to pyridoxine in vitro. Is not able to reduce 4-pyridoxate, and to oxidize pyridoxine or pyridoxamine (PubMed:27941785). Has Kemp eliminase activity towards the non-physiological substrate 5-nitrobenzisoxazole, producing 4-nitro-2-cyanophenol; this activity is not considered to be physiologically relevant (PubMed:21332126).<ref>PMID:21332126</ref> <ref>PMID:27941785</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Donkor AK]]
[[Category: Musayev FN]]
[[Category: Safo MK]]

Latest revision as of 09:25, 18 December 2024

Crystal Structure of Pyridoxal Reductase (PDXI)Crystal Structure of Pyridoxal Reductase (PDXI)

Structural highlights

8te8 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PDXI_ECOLI Catalyzes the NAD(P)H-dependent reduction of pyridoxal to pyridoxine in vitro. Is not able to reduce 4-pyridoxate, and to oxidize pyridoxine or pyridoxamine (PubMed:27941785). Has Kemp eliminase activity towards the non-physiological substrate 5-nitrobenzisoxazole, producing 4-nitro-2-cyanophenol; this activity is not considered to be physiologically relevant (PubMed:21332126).[1] [2]

References

  1. ↑ Khersonsky O, Malitsky S, Rogachev I, Tawfik DS. Role of chemistry versus substrate binding in recruiting promiscuous enzyme functions. Biochemistry. 2011 Apr 5;50(13):2683-90. PMID:21332126 doi:10.1021/bi101763c
  2. ↑ Sevin DC, Fuhrer T, Zamboni N, Sauer U. Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli. Nat Methods. 2017 Feb;14(2):187-194. doi: 10.1038/nmeth.4103. Epub 2016 Dec 12. PMID:27941785 doi:http://dx.doi.org/10.1038/nmeth.4103

8te8, resolution 2.20Å

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