1jl4: Difference between revisions

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-[[Image:1jl4.gif|left|200px]]<br />
-<applet load="1jl4" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1jl4, resolution 4.3&Aring;" />
-'''CRYSTAL STRUCTURE OF THE HUMAN CD4 N-TERMINAL TWO DOMAIN FRAGMENT COMPLEXED TO A CLASS II MHC MOLECULE'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE HUMAN CD4 N-TERMINAL TWO DOMAIN FRAGMENT COMPLEXED TO A CLASS II MHC MOLECULE==
-The structural basis of the interaction between the CD4 coreceptor and a, class II major histocompatibility complex (MHC) is described. The crystal, structure of a complex containing the human CD4 N-terminal two-domain, fragment and the murine I-A(k) class II MHC molecule with associated, peptide (pMHCII) shows that only the "top corner" of the CD4 molecule, directly contacts pMHCII. The CD4 Phe-43 side chain extends into a, hydrophobic concavity formed by MHC residues from both alpha 2 and beta 2, domains. A ternary model of the CD4-pMHCII-T-cell receptor (TCR) reveals, that the complex appears V-shaped with the membrane-proximal pMHCII at the, apex. This configuration excludes a direct TCR-CD4 interaction and, suggests how TCR and CD4 signaling is coordinated around the antigenic, pMHCII complex. Human CD4 binds to HIV gp120 in a manner strikingly, similar to the way in which CD4 interacts with pMHCII. Additional contacts, between gp120 and CD4 give the CD4-gp120 complex a greater affinity. Thus, ligation of the viral envelope glycoprotein to CD4 occludes the, pMHCII-binding site on CD4, contributing to immunodeficiency.
+<StructureSection load='1jl4' size='340' side='right'caption='[[1jl4]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1jl4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The March 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''T-Cell Receptor''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_3 10.2210/rcsb_pdb/mom_2005_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JL4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl4 OCA], [https://pdbe.org/1jl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jl4 RCSB], [https://www.ebi.ac.uk/pdbsum/1jl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jl4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HA2K_MOUSE HA2K_MOUSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jl4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jl4 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The structural basis of the interaction between the CD4 coreceptor and a class II major histocompatibility complex (MHC) is described. The crystal structure of a complex containing the human CD4 N-terminal two-domain fragment and the murine I-A(k) class II MHC molecule with associated peptide (pMHCII) shows that only the "top corner" of the CD4 molecule directly contacts pMHCII. The CD4 Phe-43 side chain extends into a hydrophobic concavity formed by MHC residues from both alpha 2 and beta 2 domains. A ternary model of the CD4-pMHCII-T-cell receptor (TCR) reveals that the complex appears V-shaped with the membrane-proximal pMHCII at the apex. This configuration excludes a direct TCR-CD4 interaction and suggests how TCR and CD4 signaling is coordinated around the antigenic pMHCII complex. Human CD4 binds to HIV gp120 in a manner strikingly similar to the way in which CD4 interacts with pMHCII. Additional contacts between gp120 and CD4 give the CD4-gp120 complex a greater affinity. Thus, ligation of the viral envelope glycoprotein to CD4 occludes the pMHCII-binding site on CD4, contributing to immunodeficiency.
-==About this Structure==
+Crystal structure of the human CD4 N-terminal two-domain fragment complexed to a class II MHC molecule.,Wang JH, Meijers R, Xiong Y, Liu JH, Sakihama T, Zhang R, Joachimiak A, Reinherz EL Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10799-804. Epub 2001 Sep 4. PMID:11535811<ref>PMID:11535811</ref>
-JL4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JL4 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of the human CD4 N-terminal two-domain fragment complexed to a class II MHC molecule., Wang JH, Meijers R, Xiong Y, Liu JH, Sakihama T, Zhang R, Joachimiak A, Reinherz EL, Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10799-804. Epub 2001 Sep 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11535811 11535811]
+</div>
+<div class="pdbe-citations 1jl4" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[CD4 3D structures|CD4 3D structures]]
+*[[MHC 3D structures|MHC 3D structures]]
+*[[MHC II 3D structures|MHC II 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Gallus gallus]]
 [[Category: Homo sapiens]]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: RCSB PDB Molecule of the Month]]
-[[Category: Meijers, R.]]
+[[Category: T-Cell Receptor]]
-[[Category: Reinherz, E.L.]]
+[[Category: Meijers R]]
-[[Category: Wang, J.H.]]
+[[Category: Reinherz EL]]
-[[Category: protein-protein complex]]
+[[Category: Wang J-H]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:42:02 2007''