4v5a: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4v5a]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v46 2v46], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v47 2v47], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v48 2v48] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v49 2v49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V5A FirstGlance]. <br> | <table><tr><td colspan='2'>[[4v5a]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v46 2v46], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v47 2v47], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v48 2v48] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v49 2v49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V5A FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v5a OCA], [https://pdbe.org/4v5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v5a RCSB], [https://www.ebi.ac.uk/pdbsum/4v5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v5a ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v5a OCA], [https://pdbe.org/4v5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v5a RCSB], [https://www.ebi.ac.uk/pdbsum/4v5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v5a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
Latest revision as of 13:40, 10 January 2024
Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMetStructure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet
Structural highlights
FunctionRS2_THET8 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B] Publication Abstract from PubMedIn bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a stop codon in the A site, a transfer RNA anticodon stem-loop in the P site and tRNA(fMet) in the E site. The work demonstrates that structures of translation factors bound to 70S ribosomes can be determined at reasonably high resolution. Contrary to earlier reports, we did not observe any RRF-induced changes in bridges connecting the two subunits. This suggests that such changes are not a direct requirement for or consequence of RRF binding but possibly arise from the subsequent stabilization of a hybrid state of the ribosome. Crystal structure of the ribosome recycling factor bound to the ribosome.,Weixlbaumer A, Petry S, Dunham CM, Selmer M, Kelley AC, Ramakrishnan V Nat Struct Mol Biol. 2007 Aug;14(8):733-7. Epub 2007 Jul 29. PMID:17660830[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also
References
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