7ygd: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7ygd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YGD FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ygd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YGD FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.41Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ygd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ygd OCA], [https://pdbe.org/7ygd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ygd RCSB], [https://www.ebi.ac.uk/pdbsum/7ygd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ygd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ygd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ygd OCA], [https://pdbe.org/7ygd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ygd RCSB], [https://www.ebi.ac.uk/pdbsum/7ygd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ygd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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</div> | </div> | ||
<div class="pdbe-citations 7ygd" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 7ygd" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Ribozyme 3D structures|Ribozyme 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Latest revision as of 10:32, 3 July 2024
Cryo-EM structure of Tetrahymena ribozyme conformation 6 undergoing the second-step self-splicingCryo-EM structure of Tetrahymena ribozyme conformation 6 undergoing the second-step self-splicing
Structural highlights
Publication Abstract from PubMedGroup I introns are catalytic RNAs that coordinate two consecutive transesterification reactions for self-splicing. To understand how the group I intron promotes catalysis and coordinates self-splicing reactions, we determine the structures of L-16 Tetrahymena ribozyme in complex with a 5'-splice site analog product and a 3'-splice site analog substrate using cryo-EM. We solve six conformations from a single specimen, corresponding to different splicing intermediates after the first ester-transfer reaction. The structures reveal dynamics during self-splicing, including large conformational changes of the internal guide sequence and the J5/4 junction as well as subtle rearrangements of active-site metals and the hydrogen bond formed between the 2'-OH group of A261 and the N2 group of guanosine substrate. These results help complete a detailed structural and mechanistic view of this paradigmatic group I intron undergoing the second step of self-splicing. Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.,Li S, Palo MZ, Zhang X, Pintilie G, Zhang K Nat Commun. 2023 Mar 16;14(1):1294. doi: 10.1038/s41467-023-36724-5. PMID:36928031[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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