2mlg: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/O05472_SULIS O05472_SULIS]  
[https://www.uniprot.org/uniprot/O05472_SULIS O05472_SULIS]  
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== Publication Abstract from PubMed ==
The hybrid plasmid-virus pSSVx from Sulfolobus islandicus presents an open reading frame encoding a 76 amino acid protein, namely Stf76, that does not show significant sequence homology with any protein with known 3D structure. The recombinant protein recognizes specifically two DNA-binding sites located in its own promoter, thus suggesting an auto-regulated role of its expression. Circular dichroism, spectrofluorimetric, light scattering and isothermal titration calorimetry experiments indicated a 2:1 molar ratio (protein:DNA) upon binding to the DNA target containing a single site. Furthermore, the solution structure of Stf76, determined by nuclear magnetic resonance (NMR) using chemical shift Rosetta software, has shown that the protein assumes a winged helix-turn-helix fold. NMR chemical shift perturbation analysis has been performed for the identification of the residues responsible for DNA interaction. In addition, a model of the Stf76-DNA complex has been built using as template a structurally related homolog.
Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.,Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E Nucleic Acids Res. 2014 May 1;42(9):5993-6011. doi: 10.1093/nar/gku215. Epub 2014, Mar 25. PMID:24682827<ref>PMID:24682827</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mlg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
</StructureSection>

Latest revision as of 10:00, 1 May 2024

Stf76 from the Sulfolobus islandicus plasmid-virus pSSVxStf76 from the Sulfolobus islandicus plasmid-virus pSSVx

Structural highlights

2mlg is a 1 chain structure with sequence from Fuselloviridae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O05472_SULIS

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