8bqs: Difference between revisions

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'''Unreleased structure'''


The entry 8bqs is ON HOLD
==Cryo-EM structure of the I-II-III2-IV2 respiratory supercomplex from Tetrahymena thermophila==
<StructureSection load='8bqs' size='340' side='right'caption='[[8bqs]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8bqs]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila_SB210 Tetrahymena thermophila SB210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BQS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=8Q1:~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl]+dodecanethioate'>8Q1</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LPP:2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL+HEXADECANOATE'>LPP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UQ8:UBIQUINONE-8'>UQ8</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bqs OCA], [https://pdbe.org/8bqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bqs RCSB], [https://www.ebi.ac.uk/pdbsum/8bqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bqs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q22E24_TETTS Q22E24_TETTS]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane(1). Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III(2)-IV(2) supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.


Authors: Muhleip, A., Kock Flygaard, R., Baradaran, R., Amunts, A.
Structural basis of mitochondrial membrane bending by the I-II-III(2)-IV(2) supercomplex.,Muhleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Marechal A, Sharma V, Amunts A Nature. 2023 Mar;615(7954):934-938. doi: 10.1038/s41586-023-05817-y. Epub 2023 , Mar 22. PMID:36949187<ref>PMID:36949187</ref>


Description: Cryo-EM structure of the I-II-III2-IV2 respiratory supercomplex from Tetrahymena thermophila
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Kock Flygaard, R]]
<div class="pdbe-citations 8bqs" style="background-color:#fffaf0;"></div>
[[Category: Amunts, A]]
 
[[Category: Baradaran, R]]
==See Also==
[[Category: Muhleip, A]]
*[[Succinate dehydrogenase 3D structures|Succinate dehydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tetrahymena thermophila SB210]]
[[Category: Amunts A]]
[[Category: Baradaran R]]
[[Category: Kock Flygaard R]]
[[Category: Muhleip A]]

Latest revision as of 18:00, 19 February 2025

Cryo-EM structure of the I-II-III2-IV2 respiratory supercomplex from Tetrahymena thermophilaCryo-EM structure of the I-II-III2-IV2 respiratory supercomplex from Tetrahymena thermophila

Structural highlights

8bqs is a 10 chain structure with sequence from Tetrahymena thermophila SB210. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.9Å
Ligands:, , , , , , , , , , , , , , , , , , , , , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q22E24_TETTS

Publication Abstract from PubMed

Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane(1). Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III(2)-IV(2) supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.

Structural basis of mitochondrial membrane bending by the I-II-III(2)-IV(2) supercomplex.,Muhleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Marechal A, Sharma V, Amunts A Nature. 2023 Mar;615(7954):934-938. doi: 10.1038/s41586-023-05817-y. Epub 2023 , Mar 22. PMID:36949187[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Mühleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Maréchal A, Sharma V, Amunts A. Structural basis of mitochondrial membrane bending by the I-II-III(2)-IV(2) supercomplex. Nature. 2023 Mar;615(7954):934-938. PMID:36949187 doi:10.1038/s41586-023-05817-y

8bqs, resolution 2.90Å

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