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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2lmc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LMC FirstGlance]. <br>
<table><tr><td colspan='2'>[[2lmc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LMC FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lmc OCA], [https://pdbe.org/2lmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lmc RCSB], [https://www.ebi.ac.uk/pdbsum/2lmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lmc ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lmc OCA], [https://pdbe.org/2lmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lmc RCSB], [https://www.ebi.ac.uk/pdbsum/2lmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lmc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/VRPI_BPT7 VRPI_BPT7] Bacterial RNA polymerase inhibitor, which is expressed in the late stage of lytic development, is also essential for continuous synthesis of phage DNA and functions in the packaging of the viral genome into the mature phage particle.
[https://www.uniprot.org/uniprot/VRPI_BPT7 VRPI_BPT7] Bacterial RNA polymerase inhibitor, which is expressed in the late stage of lytic development, is also essential for continuous synthesis of phage DNA and functions in the packaging of the viral genome into the mature phage particle.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The T7 phage-encoded small protein Gp2 is a non-DNA-binding transcription factor that interacts with the jaw domain of the Escherichia coli (Ec) RNA polymerase (RNAp) beta' subunit and inhibits transcriptionally proficient promoter-complex (RPo) formation. Here, we describe the high-resolution solution structure of the Gp2-Ec beta' jaw domain complex and show that Gp2 and DNA compete for binding to the beta' jaw domain. We reveal that efficient inhibition of RPo formation by Gp2 requires the amino-terminal sigma(70) domain region 1.1 (R1.1), and that Gp2 antagonizes the obligatory movement of R1.1 during RPo formation. We demonstrate that Gp2 inhibits RPo formation not just by steric occlusion of the RNAp-DNA interaction but also through long-range antagonistic effects on RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of R1.1 by Gp2. The inhibition of Ec RNAp by Gp2 thus defines a previously uncharacterized mechanism by which bacterial transcription is regulated by a viral factor.
Structural and Mechanistic Basis for the Inhibition of Escherichia coli RNA Polymerase by T7 Gp2.,James E, Liu M, Sheppard C, Mekler V, Camara B, Liu B, Simpson P, Cota E, Severinov K, Matthews S, Wigneshweraraj S Mol Cell. 2012 Jul 19. PMID:22819324<ref>PMID:22819324</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lmc" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
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</StructureSection>
</StructureSection>

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