7ypq: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7ypq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera_nucleopolyhedrovirus Helicoverpa armigera nucleopolyhedrovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ypq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera_nucleopolyhedrovirus Helicoverpa armigera nucleopolyhedrovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPQ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypq OCA], [https://pdbe.org/7ypq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypq RCSB], [https://www.ebi.ac.uk/pdbsum/7ypq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypq ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypq OCA], [https://pdbe.org/7ypq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypq RCSB], [https://www.ebi.ac.uk/pdbsum/7ypq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypq ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
Latest revision as of 10:34, 3 July 2024
Cryo-EM structure of one baculovirus LEF-3 molecule in complex with ssDNACryo-EM structure of one baculovirus LEF-3 molecule in complex with ssDNA
Structural highlights
FunctionPublication Abstract from PubMedSingle-stranded DNA-binding proteins (SSBs) interact with single-stranded DNA (ssDNA) to form filamentous structures with various degrees of cooperativity, as a result of intermolecular interactions between neighboring SSB subunits on ssDNA. However, it is still challenging to perform structural studies on SSB-ssDNA filaments at high resolution using the most studied SSB models, largely due to the intrinsic flexibility of these nucleoprotein complexes. In this study, HaLEF-3, an SSB protein from Helicoverpa armigera nucleopolyhedrovirus, was used for in vitro assembly of SSB-ssDNA filaments, which were structurally studied at atomic resolution using cryo-electron microscopy. Combined with the crystal structure of ssDNA-free HaLEF-3 octamers, our results revealed that the three-dimensional rearrangement of HaLEF-3 induced by an internal hinge-bending movement is essential for the formation of helical SSB-ssDNA complexes, while the contacting interface between adjacent HaLEF-3 subunits remains basically intact. We proposed a local cooperative SSB-ssDNA binding model, in which, triggered by exposure to oligonucleotides, HaLEF-3 molecules undergo ring-to-helix transition to initiate continuous SSB-SSB interactions along ssDNA. Unique structural features revealed by the assembly of HaLEF-3 on ssDNA suggest that HaLEF-3 may represent a new class of SSB. Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA.,Yin J, Fu Y, Rao G, Li Z, Tian K, Chong T, Kuang K, Wang M, Hu Z, Cao S Nucleic Acids Res. 2022 Dec 9;50(22):13100-13113. doi: 10.1093/nar/gkac1142. PMID:36477586[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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