8c4t: Difference between revisions
New page: '''Unreleased structure''' The entry 8c4t is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Hantaan virus polymerase bound to its 5' viral RNA== | |||
<StructureSection load='8c4t' size='340' side='right'caption='[[8c4t]], [[Resolution|resolution]] 3.23Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8c4t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hantaan_virus_76-118 Hantaan virus 76-118]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C4T FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.23Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c4t OCA], [https://pdbe.org/8c4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c4t RCSB], [https://www.ebi.ac.uk/pdbsum/8c4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c4t ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/L_HANTV L_HANTV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hantaviruses are causing life-threatening zoonotic infections in humans. Their tripartite negative-stranded RNA genome is replicated by the multi-functional viral RNA-dependent RNA-polymerase. Here we describe the structure of the Hantaan virus polymerase core and establish conditions for in vitro replication activity. The apo structure adopts an inactive conformation that involves substantial folding rearrangement of polymerase motifs. Binding of the 5' viral RNA promoter triggers Hantaan virus polymerase reorganization and activation. It induces the recruitment of the 3' viral RNA towards the polymerase active site for prime-and-realign initiation. The elongation structure reveals the formation of a template/product duplex in the active site cavity concomitant with polymerase core widening and the opening of a 3' viral RNA secondary binding site. Altogether, these elements reveal the molecular specificities of Hantaviridae polymerase structure and uncover the mechanisms underlying replication. They provide a solid framework for future development of antivirals against this group of emerging pathogens. | |||
Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.,Durieux Trouilleton Q, Barata-Garcia S, Arragain B, Reguera J, Malet H Nat Commun. 2023 May 23;14(1):2954. doi: 10.1038/s41467-023-38555-w. PMID:37221161<ref>PMID:37221161</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8c4t" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hantaan virus 76-118]] | |||
[[Category: Large Structures]] | |||
[[Category: Arragain B]] | |||
[[Category: Durieux trouilleton Q]] | |||
[[Category: Malet H]] | |||
Latest revision as of 09:49, 24 July 2024
Hantaan virus polymerase bound to its 5' viral RNAHantaan virus polymerase bound to its 5' viral RNA
Structural highlights
FunctionPublication Abstract from PubMedHantaviruses are causing life-threatening zoonotic infections in humans. Their tripartite negative-stranded RNA genome is replicated by the multi-functional viral RNA-dependent RNA-polymerase. Here we describe the structure of the Hantaan virus polymerase core and establish conditions for in vitro replication activity. The apo structure adopts an inactive conformation that involves substantial folding rearrangement of polymerase motifs. Binding of the 5' viral RNA promoter triggers Hantaan virus polymerase reorganization and activation. It induces the recruitment of the 3' viral RNA towards the polymerase active site for prime-and-realign initiation. The elongation structure reveals the formation of a template/product duplex in the active site cavity concomitant with polymerase core widening and the opening of a 3' viral RNA secondary binding site. Altogether, these elements reveal the molecular specificities of Hantaviridae polymerase structure and uncover the mechanisms underlying replication. They provide a solid framework for future development of antivirals against this group of emerging pathogens. Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.,Durieux Trouilleton Q, Barata-Garcia S, Arragain B, Reguera J, Malet H Nat Commun. 2023 May 23;14(1):2954. doi: 10.1038/s41467-023-38555-w. PMID:37221161[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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