8c4d: Difference between revisions

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-'''Unreleased structure'''
-The entry 8c4d is ON HOLD
+==N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium prophage genome==
+<StructureSection load='8c4d' size='340' side='right'caption='[[8c4d]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8c4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C4D FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c4d OCA], [https://pdbe.org/8c4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c4d RCSB], [https://www.ebi.ac.uk/pdbsum/8c4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c4d ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Multidrug-resistant (MDR) bacteria have become a growing threat to public health. The gram-positive Enterococcus faecium is classified by WHO as a high-priority pathogen among the global priority list of antibiotic-resistant bacteria. Peptidoglycan-degrading enzymes (PDEs), also known as enzybiotics, are useful bactericidal agents in the fight against resistant bacteria. In this work, a genome-based screening approach of the genome of E. faecium allowed the identification of a putative PDE gene with predictive amidase activity (EfAmi1; EC 3.5.1.28) in a prophage-integrated sequence. EfAmi1 is composed by two domains: a N-terminal Zn(2+)-dependent N-acetylmuramoyl-L-alanine amidase-2 (NALAA-2) domain and a C-terminal domain with unknown structure and function. The full-length gene of EfAmi1 was cloned and expressed as a 6xHis-tagged protein in E. coli. EfAmi1 was produced as a soluble protein, purified, and its lytic and antimicrobial activities were investigated using turbidity reduction and Kirby-Bauer disk-diffusion assays against clinically isolated bacterial pathogens. The crystal structure of the N-terminal amidase-2 domain was determined using X-ray crystallography at 1.97 A resolution. It adopts a globular fold with several alpha-helices surrounding a central five-stranded beta-sheet. Sequence comparison revealed a cluster of conserved amino acids that defines a putative binding site for a buried zinc ion. The results of the present study suggest that EfAmi1 displays high lytic and antimicrobial activity and may represent a promising new antimicrobial in the post-antibiotic era.
-Authors: Papageorgiou, A.C., Premetis, G.E., Labrou, N.E.
+Structural and functional features of a broad-spectrum prophage-encoded enzybiotic from Enterococcus faecium.,Premetis GE, Stathi A, Papageorgiou AC, Labrou NE Sci Rep. 2023 May 8;13(1):7450. doi: 10.1038/s41598-023-34309-2. PMID:37156923<ref>PMID:37156923</ref>
-Description: N-acetylmuramoyl-L-alanine amidase from Enterococcus faecium prophage genome
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Papageorgiou, A.C]]
+<div class="pdbe-citations 8c4d" style="background-color:#fffaf0;"></div>
-[[Category: Labrou, N.E]]
+== References ==
-[[Category: Premetis, G.E]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Enterococcus faecium]]
+[[Category: Large Structures]]
+[[Category: Labrou NE]]
+[[Category: Papageorgiou AC]]
+[[Category: Premetis GE]]