8hni: Difference between revisions
New page: '''Unreleased structure''' The entry 8hni is ON HOLD Authors: Liu, Y., Abula, A., Xiao, H., Guo, H., Li, T., Zheng, L., Chen, B., Nguyen, H., Ji, X. Description: hnRNP A2/B1 RRMs in co... |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
The entry | ==hnRNP A2/B1 RRMs in complex with telomeric DNA== | ||
<StructureSection load='8hni' size='340' side='right'caption='[[8hni]], [[Resolution|resolution]] 2.64Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8hni]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HNI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.644Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hni OCA], [https://pdbe.org/8hni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hni RCSB], [https://www.ebi.ac.uk/pdbsum/8hni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hni ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[https://www.uniprot.org/uniprot/ROA2_HUMAN ROA2_HUMAN] Inclusion body myopathy with Paget disease of bone and frontotemporal dementia. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:23455423</ref> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ROA2_HUMAN ROA2_HUMAN] Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) has been identified as a nuclear DNA sensor. Upon viral infection, hnRNP A2/B1 recognizes pathogen-derived DNA as a homodimer, which is a prerequisite for its translocation to the cytoplasm to activate the interferon response. However, the DNA binding mechanism inducing hnRNP A2/B1 homodimerization is unknown. Here, we show the crystal structure of the RNA recognition motif (RRM) of hnRNP A2/B1 in complex with a U-shaped ssDNA, which mediates the formation of a newly observed protein dimer. Our biochemical assays and mutagenesis studies confirm that the hnRNP A2/B1 homodimer forms in solution by binding to pre-generated ssDNA or dsDNA with a U-shaped bulge. These results depict a potential functional state of hnRNP A2/B1 in antiviral immunity and other cellular processes. | |||
Structural Insight Into hnRNP A2/B1 Homodimerization and DNA Recognition.,Liu Y, Abula A, Xiao H, Guo H, Li T, Zheng L, Chen B, Nguyen HC, Ji X J Mol Biol. 2022 Dec 14;435(3):167920. doi: 10.1016/j.jmb.2022.167920. PMID:36528084<ref>PMID:36528084</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8hni" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Guo | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Abula A]] | ||
[[Category: | [[Category: Chen B]] | ||
[[Category: Guo H]] | |||
[[Category: Ji X]] | |||
[[Category: Li T]] | |||
[[Category: Liu Y]] | |||
[[Category: Nguyen H]] | |||
[[Category: Xiao H]] | |||
[[Category: Zheng L]] | |||