8bc5: Difference between revisions
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The | ==Recombinant Tipula oleracea Nudivirus Polyhedrin - Selenomethionine== | ||
<StructureSection load='8bc5' size='340' side='right'caption='[[8bc5]], [[Resolution|resolution]] 1.91Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8bc5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tipula_oleracea_nudivirus Tipula oleracea nudivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BC5 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bc5 OCA], [https://pdbe.org/8bc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bc5 RCSB], [https://www.ebi.ac.uk/pdbsum/8bc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bc5 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0B4VFQ3_9VIRU A0A0B4VFQ3_9VIRU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Infectious protein crystals are an essential part of the viral lifecycle for double-stranded DNA Baculoviridae and double-stranded RNA cypoviruses. These viral protein crystals, termed occlusion bodies or polyhedra, are dense protein assemblies that form a crystalline array, encasing newly formed virions. Here, using X-ray crystallography we determine the structure of a polyhedrin from Nudiviridae. This double-stranded DNA virus family is a sister-group to the baculoviruses, whose members were thought to lack occlusion bodies. The 70-year-old sample contains a well-ordered lattice formed by a predominantly alpha-helical building block that assembles into a dense, highly interconnected protein crystal. The lattice is maintained by extensive hydrophobic and electrostatic interactions, disulfide bonds, and domain switching. The resulting lattice is resistant to most environmental stresses. Comparison of this structure to baculovirus or cypovirus polyhedra shows a distinct protein structure, crystal space group, and unit cell dimensions, however, all polyhedra utilise common principles of occlusion body assembly. | |||
Atomic structure of a nudivirus occlusion body protein determined from a 70-year-old crystal sample.,Keown JR, Crawshaw AD, Trincao J, Carrique L, Gildea RJ, Horrell S, Warren AJ, Axford D, Owen R, Evans G, Bezier A, Metcalf P, Grimes JM Nat Commun. 2023 Jul 13;14(1):4160. doi: 10.1038/s41467-023-39819-1. PMID:37443157<ref>PMID:37443157</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8bc5" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Polyhedrin|Polyhedrin]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tipula oleracea nudivirus]] | |||
[[Category: Grimes JM]] | |||
[[Category: Keown JR]] | |||
Latest revision as of 14:55, 23 October 2024
Recombinant Tipula oleracea Nudivirus Polyhedrin - SelenomethionineRecombinant Tipula oleracea Nudivirus Polyhedrin - Selenomethionine
Structural highlights
FunctionPublication Abstract from PubMedInfectious protein crystals are an essential part of the viral lifecycle for double-stranded DNA Baculoviridae and double-stranded RNA cypoviruses. These viral protein crystals, termed occlusion bodies or polyhedra, are dense protein assemblies that form a crystalline array, encasing newly formed virions. Here, using X-ray crystallography we determine the structure of a polyhedrin from Nudiviridae. This double-stranded DNA virus family is a sister-group to the baculoviruses, whose members were thought to lack occlusion bodies. The 70-year-old sample contains a well-ordered lattice formed by a predominantly alpha-helical building block that assembles into a dense, highly interconnected protein crystal. The lattice is maintained by extensive hydrophobic and electrostatic interactions, disulfide bonds, and domain switching. The resulting lattice is resistant to most environmental stresses. Comparison of this structure to baculovirus or cypovirus polyhedra shows a distinct protein structure, crystal space group, and unit cell dimensions, however, all polyhedra utilise common principles of occlusion body assembly. Atomic structure of a nudivirus occlusion body protein determined from a 70-year-old crystal sample.,Keown JR, Crawshaw AD, Trincao J, Carrique L, Gildea RJ, Horrell S, Warren AJ, Axford D, Owen R, Evans G, Bezier A, Metcalf P, Grimes JM Nat Commun. 2023 Jul 13;14(1):4160. doi: 10.1038/s41467-023-39819-1. PMID:37443157[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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