6tf4: Difference between revisions

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 <StructureSection load='6tf4' size='340' side='right'caption='[[6tf4]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6tf4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TF4 FirstGlance]. <br>
+<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TF4 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tf4 OCA], [https://pdbe.org/6tf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tf4 RCSB], [https://www.ebi.ac.uk/pdbsum/6tf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tf4 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tf4 OCA], [https://pdbe.org/6tf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tf4 RCSB], [https://www.ebi.ac.uk/pdbsum/6tf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tf4 ProSAT]</span></td></tr>
 </table>
-== Function ==
-[https://www.uniprot.org/uniprot/Q9KTB3_VIBCH Q9KTB3_VIBCH] Enhances distal genes transcription elongation in a specialized subset of operons that encode extracytoplasmic components.[HAMAP-Rule:MF_00951]
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-The two-domain protein RfaH, a paralog of the universally conserved NusG/Spt5 transcription factors, is regulated by autoinhibition coupled to the reversible conformational switch of its 60-residue C-terminal Kyrpides, Ouzounis, Woese (KOW) domain between an alpha-hairpin and a beta-barrel. In contrast, NusG/Spt5-KOW domains only occur in the beta-barrel state. To understand the principles underlying the drastic fold switch in RfaH, we elucidated the thermodynamic stability and the structural dynamics of two RfaH- and four NusG/Spt5-KOW domains by combining biophysical and structural biology methods. We find that the RfaH-KOW beta-barrel is thermodynamically less stable than that of most NusG/Spt5-KOWs and we show that it is in equilibrium with a globally unfolded species, which, strikingly, contains two helical regions that prime the transition toward the alpha-hairpin. Our results suggest that transiently structured elements in the unfolded conformation might drive the global folding transition in metamorphic proteins in general.
-Structural and thermodynamic analyses of the beta-to-alpha transformation in RfaH reveal principles of fold-switching proteins.,Zuber PK, Daviter T, Heissmann R, Persau U, Schweimer K, Knauer SH Elife. 2022 Oct 18;11:e76630. doi: 10.7554/eLife.76630. PMID:36255050<ref>PMID:36255050</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 6tf4" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Vibrio cholerae]]
 [[Category: Knauer SH]]
 [[Category: Schweimer K]]
 [[Category: Zuber PK]]