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[[Image:1ihv.gif|left|200px]]
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{{STRUCTURE_1ihv|  PDB=1ihv  |  SCENE=  }}
'''SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE'''


==SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE==
<StructureSection load='1ihv' size='340' side='right'caption='[[1ihv]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ihv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IHV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ihv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ihv OCA], [https://pdbe.org/1ihv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ihv RCSB], [https://www.ebi.ac.uk/pdbsum/1ihv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ihv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/POL_HV1Z6 POL_HV1Z6] Integrase performs the integration of the newly synthesized dsDNA copy of the viral genome into the host chromosome. The integrated DNA is called provirus.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.


==Overview==
Solution structure of the DNA binding domain of HIV-1 integrase.,Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683<ref>PMID:7632683</ref>
The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1IHV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHV OCA].
</div>
<div class="pdbe-citations 1ihv" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Solution structure of the DNA binding domain of HIV-1 integrase., Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM, Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7632683 7632683]
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
[[Category: Human immunodeficiency virus]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Clore, G M.]]
__TOC__
[[Category: Ernst, J A.]]
</StructureSection>
[[Category: Gronenborn, A M.]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Lodi, P J.]]
[[Category: Large Structures]]
[[Category: Aid]]
[[Category: Clore GM]]
[[Category: Dna-binding protein]]
[[Category: Ernst JA]]
[[Category: Polyprotein]]
[[Category: Gronenborn AM]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:01:13 2008''
[[Category: Lodi PJ]]

Latest revision as of 21:41, 29 November 2023

SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURESOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE

Structural highlights

1ihv is a 2 chain structure with sequence from Human immunodeficiency virus 1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

POL_HV1Z6 Integrase performs the integration of the newly synthesized dsDNA copy of the viral genome into the host chromosome. The integrated DNA is called provirus.

Publication Abstract from PubMed

The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.

Solution structure of the DNA binding domain of HIV-1 integrase.,Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM. Solution structure of the DNA binding domain of HIV-1 integrase. Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683
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