3wwf: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3wwf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WWF FirstGlance]. <br>
<table><tr><td colspan='2'>[[3wwf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WWF FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ww7|3ww7]], [[3ww8|3ww8]], [[3ww9|3ww9]], [[3wwa|3wwa]], [[3wwb|3wwb]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.601&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wwf OCA], [https://pdbe.org/3wwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wwf RCSB], [https://www.ebi.ac.uk/pdbsum/3wwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wwf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wwf OCA], [https://pdbe.org/3wwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wwf RCSB], [https://www.ebi.ac.uk/pdbsum/3wwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wwf ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The modular structure of many protein families, such as beta-propeller proteins, strongly implies that duplication played an important role in their evolution, leading to highly symmetrical intermediate forms. Previous attempts to create perfectly symmetrical propeller proteins have failed, however. We have therefore developed a new and rapid computational approach to design such proteins. As a test case, we have created a sixfold symmetrical beta-propeller protein and experimentally validated the structure using X-ray crystallography. Each blade consists of 42 residues. Proteins carrying 2-10 identical blades were also expressed and purified. Two or three tandem blades assemble to recreate the highly stable sixfold symmetrical architecture, consistent with the duplication and fusion theory. The other proteins produce different monodisperse complexes, up to 42 blades (180 kDa) in size, which self-assemble according to simple symmetry rules. Our procedure is suitable for creating nano-building blocks from different protein templates of desired symmetry.
Computational design of a self-assembling symmetrical beta-propeller protein.,Voet AR, Noguchi H, Addy C, Simoncini D, Terada D, Unzai S, Park SY, Zhang KY, Tame JR Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15102-7. doi:, 10.1073/pnas.1412768111. Epub 2014 Oct 6. PMID:25288768<ref>PMID:25288768</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wwf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Addy, C]]
[[Category: Addy C]]
[[Category: Noguchi, H]]
[[Category: Noguchi H]]
[[Category: Park, S Y]]
[[Category: Park SY]]
[[Category: Simoncini, D]]
[[Category: Simoncini D]]
[[Category: Tame, J R.H]]
[[Category: Tame JRH]]
[[Category: Terada, D]]
[[Category: Terada D]]
[[Category: Unzai, S]]
[[Category: Unzai S]]
[[Category: Voet, A R.D]]
[[Category: Voet ARD]]
[[Category: Zhang, K Y.J]]
[[Category: Zhang KYJ]]
[[Category: Computational protein design]]
[[Category: De novo protein]]
[[Category: Self-assembly]]

Latest revision as of 11:47, 20 March 2024

Crystal structure of the computationally designed Pizza2-SR proteinCrystal structure of the computationally designed Pizza2-SR protein

Structural highlights

3wwf is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.601Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

3wwf, resolution 1.60Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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