7ypq: Difference between revisions

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-'''Unreleased structure'''
-The entry 7ypq is ON HOLD
+==Cryo-EM structure of one baculovirus LEF-3 molecule in complex with ssDNA==
+<StructureSection load='7ypq' size='340' side='right'caption='[[7ypq]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7ypq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicoverpa_armigera_nucleopolyhedrovirus Helicoverpa armigera nucleopolyhedrovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YPQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ypq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ypq OCA], [https://pdbe.org/7ypq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ypq RCSB], [https://www.ebi.ac.uk/pdbsum/7ypq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ypq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q91BW6_9ABAC Q91BW6_9ABAC]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Single-stranded DNA-binding proteins (SSBs) interact with single-stranded DNA (ssDNA) to form filamentous structures with various degrees of cooperativity, as a result of intermolecular interactions between neighboring SSB subunits on ssDNA. However, it is still challenging to perform structural studies on SSB-ssDNA filaments at high resolution using the most studied SSB models, largely due to the intrinsic flexibility of these nucleoprotein complexes. In this study, HaLEF-3, an SSB protein from Helicoverpa armigera nucleopolyhedrovirus, was used for in vitro assembly of SSB-ssDNA filaments, which were structurally studied at atomic resolution using cryo-electron microscopy. Combined with the crystal structure of ssDNA-free HaLEF-3 octamers, our results revealed that the three-dimensional rearrangement of HaLEF-3 induced by an internal hinge-bending movement is essential for the formation of helical SSB-ssDNA complexes, while the contacting interface between adjacent HaLEF-3 subunits remains basically intact. We proposed a local cooperative SSB-ssDNA binding model, in which, triggered by exposure to oligonucleotides, HaLEF-3 molecules undergo ring-to-helix transition to initiate continuous SSB-SSB interactions along ssDNA. Unique structural features revealed by the assembly of HaLEF-3 on ssDNA suggest that HaLEF-3 may represent a new class of SSB.
-Authors:
+Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA.,Yin J, Fu Y, Rao G, Li Z, Tian K, Chong T, Kuang K, Wang M, Hu Z, Cao S Nucleic Acids Res. 2022 Dec 9;50(22):13100-13113. doi: 10.1093/nar/gkac1142. PMID:36477586<ref>PMID:36477586</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7ypq" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Helicoverpa armigera nucleopolyhedrovirus]]
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Cao S]]
+[[Category: Fu Y]]
+[[Category: Li Z]]
+[[Category: Rao G]]
+[[Category: Yin J]]