7yon: Difference between revisions
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The | ==Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi== | ||
<StructureSection load='7yon' size='340' side='right'caption='[[7yon]], [[Resolution|resolution]] 2.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7yon]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YON FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TYC:L-TYROSINAMIDE'>TYC</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yon OCA], [https://pdbe.org/7yon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yon RCSB], [https://www.ebi.ac.uk/pdbsum/7yon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yon ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Neuropeptide Y (NPY) and its receptors are expressed in various human tissues including the brain where they regulate appetite and emotion. Upon NPY stimulation, the neuropeptide Y1 and Y2 receptors (Y(1)R and Y(2)R, respectively) activate G(I) signaling, but their physiological responses to food intake are different. In addition, deletion of the two N-terminal amino acids of peptide YY (PYY(3-36)), the endogenous form found in circulation, can stimulate Y(2)R but not Y(1)R, suggesting that Y(1)R and Y(2)R may have distinct ligand-binding modes. Here, we report the cryo-electron microscopy structures of the PYY(3-36)âY(2)RâG(i) and NPYâY(2)RâG(i) complexes. Using cell-based assays, molecular dynamics simulations, and structural analysis, we revealed the molecular basis of the exclusive binding of PYY(3-36) to Y(2)R. Furthermore, we demonstrated that Y(2)R favors G protein signaling over beta-arrestin signaling upon activation, whereas Y(1)R does not show a preference between these two pathways. | |||
Structural basis for Y2 receptor-mediated neuropeptide Y and peptide YY signaling.,Kang H, Park C, Choi YK, Bae J, Kwon S, Kim J, Choi C, Seok C, Im W, Choi HJ Structure. 2023 Jan 5;31(1):44-57.e6. doi: 10.1016/j.str.2022.11.010. Epub 2022 , Dec 15. PMID:36525977<ref>PMID:36525977</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7yon" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | |||
[[Category: Choi H-J]] | |||
[[Category: Kang H]] | |||
[[Category: Kim J]] | |||
[[Category: Park C]] | |||
Latest revision as of 10:17, 22 March 2023
Complex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and GiComplex structure of Neuropeptide Y Y2 receptor in complex with PYY(3-36) and Gi
Structural highlights
FunctionGNAI1_HUMAN Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.[1] [2] Publication Abstract from PubMedNeuropeptide Y (NPY) and its receptors are expressed in various human tissues including the brain where they regulate appetite and emotion. Upon NPY stimulation, the neuropeptide Y1 and Y2 receptors (Y(1)R and Y(2)R, respectively) activate G(I) signaling, but their physiological responses to food intake are different. In addition, deletion of the two N-terminal amino acids of peptide YY (PYY(3-36)), the endogenous form found in circulation, can stimulate Y(2)R but not Y(1)R, suggesting that Y(1)R and Y(2)R may have distinct ligand-binding modes. Here, we report the cryo-electron microscopy structures of the PYY(3-36)âY(2)RâG(i) and NPYâY(2)RâG(i) complexes. Using cell-based assays, molecular dynamics simulations, and structural analysis, we revealed the molecular basis of the exclusive binding of PYY(3-36) to Y(2)R. Furthermore, we demonstrated that Y(2)R favors G protein signaling over beta-arrestin signaling upon activation, whereas Y(1)R does not show a preference between these two pathways. Structural basis for Y2 receptor-mediated neuropeptide Y and peptide YY signaling.,Kang H, Park C, Choi YK, Bae J, Kwon S, Kim J, Choi C, Seok C, Im W, Choi HJ Structure. 2023 Jan 5;31(1):44-57.e6. doi: 10.1016/j.str.2022.11.010. Epub 2022 , Dec 15. PMID:36525977[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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