3wdf: Difference between revisions
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<StructureSection load='3wdf' size='340' side='right'caption='[[3wdf]], [[Resolution|resolution]] 1.48Å' scene=''> | <StructureSection load='3wdf' size='340' side='right'caption='[[3wdf]], [[Resolution|resolution]] 1.48Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wdf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3wdf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MRSA252 Staphylococcus aureus subsp. aureus MRSA252]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WDF FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wdf OCA], [https://pdbe.org/3wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wdf RCSB], [https://www.ebi.ac.uk/pdbsum/3wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wdf ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wdf OCA], [https://pdbe.org/3wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wdf RCSB], [https://www.ebi.ac.uk/pdbsum/3wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wdf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/UNG_STAAR UNG_STAAR] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity). | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Staphylococcus aureus subsp. aureus MRSA252]] | ||
[[Category: Ko TP]] | |||
[[Category: Ko | [[Category: Wang AHJ]] | ||
[[Category: Wang | [[Category: Wang HC]] | ||
[[Category: Wang | |||
Latest revision as of 16:06, 8 November 2023
Staphylococcus aureus UDGStaphylococcus aureus UDG
Structural highlights
FunctionUNG_STAAR Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity). Publication Abstract from PubMedDNA mimic proteins are unique factors that control the DNA binding activity of target proteins by directly occupying their DNA binding sites. The extremely divergent amino acid sequences of the DNA mimics make these proteins hard to predict, and although they are likely to be ubiquitous, to date, only a few have been reported and functionally analyzed. Here we used a bioinformatic approach to look for potential DNA mimic proteins among previously reported protein structures. From approximately 14 candidates, we selected the Staphylococcus conserved hypothetical protein SSP0047, and used proteomic and structural approaches to show that it is a novel DNA mimic protein. In Staphylococcus aureus, we found that this protein acts as a uracil-DNA glycosylase inhibitor, and therefore named it S. aureus uracil-DNA glycosylase inhibitor (SAUGI). We also determined and analyzed the complex structure of SAUGI and S. aureus uracil-DNA glycosylase (SAUDG). Subsequent BIAcore studies further showed that SAUGI has a high binding affinity to both S. aureus and human UDG. The two uracil-DNA glycosylase inhibitors (UGI and p56) previously known to science were both found in Bacillus phages, and this is the first report of a bacterial DNA mimic that may regulate SAUDG's functional roles in DNA repair and host defense. Staphylococcus aureus protein SAUGI acts as a uracil-DNA glycosylase inhibitor.,Wang HC, Hsu KC, Yang JM, Wu ML, Ko TP, Lin SR, Wang AH Nucleic Acids Res. 2014 Jan 1;42(2):1354-64. doi: 10.1093/nar/gkt964. Epub 2013, Oct 22. PMID:24150946[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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