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| <StructureSection load='3uib' size='340' side='right'caption='[[3uib]], [[Resolution|resolution]] 2.65Å' scene=''> | | <StructureSection load='3uib' size='340' side='right'caption='[[3uib]], [[Resolution|resolution]] 2.65Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3uib]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UIB FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3uib]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UIB FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SB2:4-[5-(4-FLUORO-PHENYL)-2-(4-METHANESULFINYL-PHENYL)-3H-IMIDAZOL-4-YL]-PYRIDINE'>SB2</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pg1|3pg1]]</div></td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SB2:4-[5-(4-FLUORO-PHENYL)-2-(4-METHANESULFINYL-PHENYL)-3H-IMIDAZOL-4-YL]-PYRIDINE'>SB2</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LMJF10.0200, LMJF_10_0200 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 LEIMA])</td></tr> | |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uib OCA], [https://pdbe.org/3uib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uib RCSB], [https://www.ebi.ac.uk/pdbsum/3uib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uib ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uib OCA], [https://pdbe.org/3uib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uib RCSB], [https://www.ebi.ac.uk/pdbsum/3uib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uib ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q4QHJ8_LEIMA Q4QHJ8_LEIMA] |
| Mitogen-activated protein kinases (MAPKs) are involved in environmental signal sensing. They are thus expected to play key roles in the biology of Trypanosomatid parasites, which display complex life cycles and use extracellular cues to modulate cell differentiation. Despite their relevance, structural data of Trypanosomatid MAPKs is lacking. We have now determined the crystal structure of Leishmania major LmaMPK10, a stage-specifically activated MAPK, both alone and in complex with SB203580. LmaMPK10 was observed to be more similar to p38 than to other human MAPKs. However, significant differences could be identified in the catalytic pocket, as well as in potentially regulatory sites in the N-terminal lobe. The modified pocket architecture in LmaMPK10 precludes DFG-in/DFG-out regulatory flipping as observed in mammalian MAPKs. LmaMPK10-nucleotide association was also studied, revealing a potential C-terminal autoinhibitory mechanism. Overall, these data should speed the discovery of molecules interfering with LmaMPK10 functions, with relevance for antileishmanial drug development strategies.
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| The Crystal Structure of the MAP Kinase LmaMPK10 from Leishmania Major Reveals Parasite-Specific Features and Regulatory Mechanisms.,Horjales S, Schmidt-Arras D, Limardo RR, Leclercq O, Obal G, Prina E, Turjanski AG, Spath GF, Buschiazzo A Structure. 2012 Aug 7. PMID:22884419<ref>PMID:22884419</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3uib" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Mitogen-activated protein kinase 3D structures|Mitogen-activated protein kinase 3D structures]] | | *[[Mitogen-activated protein kinase 3D structures|Mitogen-activated protein kinase 3D structures]] |
| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Leima]] | | [[Category: Leishmania major]] |
| [[Category: Non-specific serine/threonine protein kinase]]
| | [[Category: Buschiazzo A]] |
| [[Category: Buschiazzo, A]] | | [[Category: Horjales S]] |
| [[Category: Horjales, S]] | | [[Category: Leclercq O]] |
| [[Category: Leclercq, O]] | | [[Category: Schmidt-Arras D]] |
| [[Category: Schmidt-Arras, D]] | | [[Category: Spath G]] |
| [[Category: Spath, G]] | |
| [[Category: Eukariotic protein kinase fold]]
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| [[Category: Transferase]]
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