7xsd: Difference between revisions

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-'''Unreleased structure'''
-The entry 7xsd is ON HOLD  until Paper Publication
+==Cryo-EM structure of RuBisCO assembly intermediate RbcL8Raf18RbcX16==
+<StructureSection load='7xsd' size='340' side='right'caption='[[7xsd]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7xsd]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSD FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsd OCA], [https://pdbe.org/7xsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsd RCSB], [https://www.ebi.ac.uk/pdbsum/7xsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsd ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RBCX_NOSS1 RBCX_NOSS1] An RbcL-specific chaperone. The central cleft of the RbcX homodimer (RbcX2) binds the C-terminus of an RbcL monomer, stabilizing the C-terminus and probably preventing its reassociation with chaperonin GroEL-ES. At the same time the peripheral region of RbcX2 binds a second RbcL monomer, bridging the RbcL homodimers in the correct orientation. The RbcX2(2)-bound RbcL dimers then assemble into the RbcL8 core (RbcL8-(RbcX2)8). RbcS binding triggers the release of RbcX2.[HAMAP-Rule:MF_00855]  When rbcL-rbcX-rbcS or rbcL-rbcS were overexpressed in E.coli no change in reconstituted RuBisCO activity was observed, which suggests RbcX plays no role in RuBisCO assembly in this system (PubMed:8472962). However in PubMed:8472962 E.coli chaperones groL and groS were also overexpressed, which may compensate for lack of rbcX (Probable).<ref>PMID:8472962</ref> <ref>PMID:15564522</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+RuBisCO is the most abundant enzyme in nature, catalyzing the fixation of CO(2) in photosynthesis. Its common form consists of eight RbcL and eight RbcS subunits, the assembly of which requires a series of chaperones that include RbcX and RuBisCO accumulation factor 1 (Raf1). To understand how these RuBisCO-specific chaperones function during cyanobacterial RbcL(8)RbcS(8) (L(8)S(8)) holoenzyme formation, we solved a 3.3-A cryo-electron microscopy structure of a 32-subunit RbcL(8)Raf1(8)RbcX(16) (L(8)F(8)X(16)) assembly intermediate from Anabaena sp. PCC 7120. Comparison to the previously resolved L(8)F(8) and L(8)X(16) structures together with biochemical assays revealed that the L(8)F(8)X(16) complex forms a rather dynamic structural intermediate, favoring RbcS displacement of Raf1 and RbcX. In vitro assays further demonstrated that both Raf1 and RbcX function to regulate RuBisCO condensate formation by restricting CcmM35 binding to the stably assembled L(8)S(8) holoenzymes. Combined with previous findings, we propose a model on how Raf1 and RbcX work in concert to facilitate, and regulate, cyanobacterial RuBisCO assembly as well as disassembly of RuBisCO condensates.
-Authors: Jiang, Y.L., Xia, L.Y., Zhou, C.Z.
+Structural insights into cyanobacterial RuBisCO assembly coordinated by two chaperones Raf1 and RbcX.,Li Q, Jiang YL, Xia LY, Chen Y, Zhou CZ Cell Discov. 2022 Sep 20;8(1):93. doi: 10.1038/s41421-022-00436-9. PMID:36123352<ref>PMID:36123352</ref>
-Description: Cryo-EM structure of RuBisCO assembly intermediate RbcL8Raf18RbcX16
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Jiang, Y.L]]
+<div class="pdbe-citations 7xsd" style="background-color:#fffaf0;"></div>
-[[Category: Zhou, C.Z]]
-[[Category: Xia, L.Y]]
+==See Also==
+*[[RuBisCO 3D structures|RuBisCO 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
+[[Category: Jiang YL]]
+[[Category: Xia LY]]
+[[Category: Zhou CZ]]