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==== | ==Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs== | ||
<StructureSection load='7vqx' size='340' side='right'caption='[[7vqx]]' scene=''> | <StructureSection load='7vqx' size='340' side='right'caption='[[7vqx]], [[Resolution|resolution]] 2.74Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7vqx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQX FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vqx OCA], [https://pdbe.org/7vqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vqx RCSB], [https://www.ebi.ac.uk/pdbsum/7vqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vqx ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.74Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vqx OCA], [https://pdbe.org/7vqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vqx RCSB], [https://www.ebi.ac.uk/pdbsum/7vqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vqx ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/GNAS2_BOVIN GNAS2_BOVIN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Class B1 of G protein-coupled receptors (GPCRs) comprises 15 members activated by physiologically important peptide hormones. Among them, vasoactive intestinal polypeptide receptor 2 (VIP2R) is expressed in the central and peripheral nervous systems and involved in a number of pathophysiological conditions, including pulmonary arterial hypertension, autoimmune and psychiatric disorders, in which it is thus a valuable drug target. Here, we report the cryo-electron microscopy structure of the human VIP2R bound to its endogenous ligand PACAP27 and the stimulatory G protein. Different from all reported peptide-bound class B1 GPCR structures, the N-terminal alpha-helix of VIP2R adopts a unique conformation that deeply inserts into a cleft between PACAP27 and the extracellular loop 1, thereby stabilizing the peptide-receptor interface. Its truncation or extension significantly decreased VIP2R-mediated cAMP accumulation. Our results provide additional information on peptide recognition and receptor activation among class B1 GPCRs and may facilitate the design of better therapeutics. | |||
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2.,Xu Y, Feng W, Zhou Q, Liang A, Li J, Dai A, Zhao F, Yan J, Chen CW, Li H, Zhao LH, Xia T, Jiang Y, Xu HE, Yang D, Wang MW Nat Commun. 2022 Apr 27;13(1):2272. doi: 10.1038/s41467-022-30041-z. PMID:35477937<ref>PMID:35477937</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7vqx" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Transducin 3D structures|Transducin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bos taurus]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Rattus norvegicus]] | ||
[[Category: Synthetic construct]] | |||
[[Category: Chen CW]] | |||
[[Category: Dai AT]] | |||
[[Category: Feng WB]] | |||
[[Category: Jiang Y]] | |||
[[Category: Li H]] | |||
[[Category: Li J]] | |||
[[Category: Liang AY]] | |||
[[Category: Wang MW]] | |||
[[Category: Xia T]] | |||
[[Category: Xu HE]] | |||
[[Category: Xu YN]] | |||
[[Category: Yan JH]] | |||
[[Category: Yang DH]] | |||
[[Category: Zhao FH]] | |||
[[Category: Zhao LH]] | |||
[[Category: Zhou QT]] | |||