7ejh: Difference between revisions

Latest revision as of 19:55, 29 November 2023

Crystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complexCrystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complex

Structural highlights

7ejh is a 4 chain structure with sequence from Lentilactobacillus kefiri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7288393Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RADH_LENKE NADP-dependent (R)-specific alcohol dehydrogenase (ADH) with a broad substrate specificity, able to catalyze in vitro the stereoselective reduction of several aliphatic and aromatic ketones as well as beta-keto esters to the corresponding enantiomerically pure alcohols.^[1]

References

↑ Noey EL, Tibrewal N, Jimenez-Oses G, Osuna S, Park J, Bond CM, Cascio D, Liang J, Zhang X, Huisman GW, Tang Y, Houk KN. Origins of stereoselectivity in evolved ketoreductases. Proc Natl Acad Sci U S A. 2015 Dec 7. pii: 201507910. PMID:26644568 doi:http://dx.doi.org/10.1073/pnas.1507910112

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OCA

[1] Noey EL, Tibrewal N, Jimenez-Oses G, Osuna S, Park J, Bond CM, Cascio D, Liang J, Zhang X, Huisman GW, Tang Y, Houk KN. Origins of stereoselectivity in evolved ketoreductases. Proc Natl Acad Sci U S A. 2015 Dec 7. pii: 201507910. PMID:26644568 doi:http://dx.doi.org/10.1073/pnas.1507910112

[1]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 7ejh is ON HOLD  until May 06 2023
+==Crystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complex==
+<StructureSection load='7ejh' size='340' side='right'caption='[[7ejh]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7ejh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentilactobacillus_kefiri Lentilactobacillus kefiri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EJH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7288393&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=J66:2-oxidanylisoindole-1,3-dione'>J66</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ejh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ejh OCA], [https://pdbe.org/7ejh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ejh RCSB], [https://www.ebi.ac.uk/pdbsum/7ejh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ejh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RADH_LENKE RADH_LENKE] NADP-dependent (R)-specific alcohol dehydrogenase (ADH) with a broad substrate specificity, able to catalyze in vitro the stereoselective reduction of several aliphatic and aromatic ketones as well as beta-keto esters to the corresponding enantiomerically pure alcohols.<ref>PMID:26644568</ref>
-Authors: Cui, J., Huang, X., Wang, B., Zhao, H., Zhou, J.
+==See Also==
+*[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]]
-Description: Crystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complex
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
-[[Category: Huang, X]]
+__TOC__
-[[Category: Wang, B]]
+</StructureSection>
-[[Category: Zhao, H]]
+[[Category: Large Structures]]
-[[Category: Zhou, J]]
+[[Category: Lentilactobacillus kefiri]]
-[[Category: Cui, J]]
+[[Category: Cui J]]
+[[Category: Huang X]]
+[[Category: Wang B]]
+[[Category: Zhao H]]
+[[Category: Zhou J]]

7ejh: Difference between revisions

Latest revision as of 19:55, 29 November 2023

Crystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complexCrystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complex

Structural highlights

Function

See Also

References

Contents

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7ejh: Difference between revisions

Latest revision as of 19:55, 29 November 2023

Crystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complexCrystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F and 2-hydroxyisoindoline-1,3-dione complex

Structural highlights

Function

See Also

References

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