7x9r: Difference between revisions
New page: '''Unreleased structure''' The entry 7x9r is ON HOLD Authors: Description: Category: Unreleased Structures |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Crystal structure of the antirepressor GmaR== | ||
<StructureSection load='7x9r' size='340' side='right'caption='[[7x9r]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7x9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X9R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x9r OCA], [https://pdbe.org/7x9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x9r RCSB], [https://www.ebi.ac.uk/pdbsum/7x9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x9r ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8Y949_LISMO Q8Y949_LISMO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The pathogenic Listeria monocytogenes bacterium produces the flagellum as a locomotive organelle at or below 30 degrees C outside the host, but it halts flagellar expression at 37 degrees C inside the human host to evade the flagellum-induced immune response. Listeria monocytogenes GmaR is a thermosensor protein that coordinates flagellar expression by binding the master transcriptional repressor of flagellar genes (MogR) in a temperature-responsive manner. To understand the regulatory mechanism whereby GmaR exerts the antirepression activity on flagellar expression, we performed structural and mutational analyses of the GmaR-MogR system. At or below 30 degrees C, GmaR exists as a functional monomer and forms a circularly enclosed multidomain structure via an interdomain interaction. GmaR in this conformation recognizes MogR using the C-terminal antirepressor domain in a unique dual binding mode and mediates the antirepressor function through direct competition and spatial restraint mechanisms. Surprisingly, at 37 degrees C, GmaR rapidly forms autologous aggregates that are deficient in MogR neutralization capabilities. | |||
Structural basis of flagellar motility regulation by the MogR repressor and the GmaR antirepressor in Listeria monocytogenes.,Cho SY, Na HW, Oh HB, Kwak YM, Song WS, Park SC, Jeon WJ, Cho H, Oh BC, Park J, Kang SG, Lee GS, Yoon SI Nucleic Acids Res. 2022 Oct 28;50(19):11315-11330. doi: 10.1093/nar/gkac815. PMID:36283692<ref>PMID:36283692</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7x9r" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Listeria monocytogenes]] | |||
[[Category: Cho SY]] | |||
[[Category: Kwak YM]] | |||
[[Category: Na HW]] | |||
[[Category: Oh HB]] | |||
[[Category: Park SC]] | |||
[[Category: Song WS]] | |||
[[Category: Yoon SI]] | |||
Latest revision as of 22:45, 29 May 2024
Crystal structure of the antirepressor GmaRCrystal structure of the antirepressor GmaR
Structural highlights
FunctionPublication Abstract from PubMedThe pathogenic Listeria monocytogenes bacterium produces the flagellum as a locomotive organelle at or below 30 degrees C outside the host, but it halts flagellar expression at 37 degrees C inside the human host to evade the flagellum-induced immune response. Listeria monocytogenes GmaR is a thermosensor protein that coordinates flagellar expression by binding the master transcriptional repressor of flagellar genes (MogR) in a temperature-responsive manner. To understand the regulatory mechanism whereby GmaR exerts the antirepression activity on flagellar expression, we performed structural and mutational analyses of the GmaR-MogR system. At or below 30 degrees C, GmaR exists as a functional monomer and forms a circularly enclosed multidomain structure via an interdomain interaction. GmaR in this conformation recognizes MogR using the C-terminal antirepressor domain in a unique dual binding mode and mediates the antirepressor function through direct competition and spatial restraint mechanisms. Surprisingly, at 37 degrees C, GmaR rapidly forms autologous aggregates that are deficient in MogR neutralization capabilities. Structural basis of flagellar motility regulation by the MogR repressor and the GmaR antirepressor in Listeria monocytogenes.,Cho SY, Na HW, Oh HB, Kwak YM, Song WS, Park SC, Jeon WJ, Cho H, Oh BC, Park J, Kang SG, Lee GS, Yoon SI Nucleic Acids Res. 2022 Oct 28;50(19):11315-11330. doi: 10.1093/nar/gkac815. PMID:36283692[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||