7r6r: Difference between revisions

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-'''Unreleased structure'''
-The entry 7r6r is ON HOLD  until Paper Publication
+==Crystal Structure of a Mycobacteriophage Cluster A2 Immunity Repressor:DNA Complex==
+<StructureSection load='7r6r' size='340' side='right'caption='[[7r6r]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7r6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_phage_TipsytheTRex Mycobacterium phage TipsytheTRex]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7R6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7R6R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.13&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7r6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7r6r OCA], [https://pdbe.org/7r6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7r6r RCSB], [https://www.ebi.ac.uk/pdbsum/7r6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7r6r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A2D1GKF7_9CAUD A0A2D1GKF7_9CAUD]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Regulation of bacteriophage gene expression involves repressor proteins that bind and downregulate early lytic promoters. A large group of mycobacteriophages code for repressors that are unusual in also terminating transcription elongation at numerous binding sites (stoperators) distributed across the phage genome. Here we provide the X-ray crystal structure of a mycobacteriophage immunity repressor bound to DNA, which reveals the binding of a monomer to an asymmetric DNA sequence using two independent DNA binding domains. The structure is supported by small-angle X-ray scattering, DNA binding, molecular dynamics, and in vivo immunity assays. We propose a model for how dual DNA binding domains facilitate regulation of both transcription initiation and elongation, while enabling evolution of other superinfection immune specificities.
-Authors:
+A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence.,McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR Nat Commun. 2022 Jul 14;13(1):4105. doi: 10.1038/s41467-022-31678-6. PMID:35835745<ref>PMID:35835745</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 7r6r" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mycobacterium phage TipsytheTRex]]
+[[Category: Brambley CA]]
+[[Category: Cafferty ER]]
+[[Category: Gainey MD]]
+[[Category: Gragg KN]]
+[[Category: Green WC]]
+[[Category: Hammel M]]
+[[Category: Hollis TJ]]
+[[Category: McGinnis RJ]]
+[[Category: Miller JM]]
+[[Category: Stamey B]]
+[[Category: Terwilliger TC]]
+[[Category: Wallen JR]]