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| <StructureSection load='7rsl' size='340' side='right'caption='[[7rsl]], [[Resolution|resolution]] 3.45Å' scene=''> | | <StructureSection load='7rsl' size='340' side='right'caption='[[7rsl]], [[Resolution|resolution]] 3.45Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7rsl]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RSL FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RSL FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsl OCA], [https://pdbe.org/7rsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rsl RCSB], [https://www.ebi.ac.uk/pdbsum/7rsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rsl ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.45Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsl OCA], [https://pdbe.org/7rsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rsl RCSB], [https://www.ebi.ac.uk/pdbsum/7rsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rsl ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
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| [[https://www.uniprot.org/uniprot/SEI1_YEAST SEI1_YEAST]] Involved in lipid metabolism and lipid droplet (LD) morphology, number, and size (PubMed:18093937, PubMed:18250201). Facilitates initiation of LD formation, and ensures that vectorial budding of LDs from the ER is directed towards the cytoplasm (PubMed:25540432).<ref>PMID:18093937</ref> <ref>PMID:18250201</ref> <ref>PMID:25540432</ref>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Lipid droplets (LDs) form in the endoplasmic reticulum by phase separation of neutral lipids. This process is facilitated by the seipin protein complex, which consists of a ring of seipin monomers, with a yet unclear function. Here, we report a structure of S. cerevisiae seipin based on cryogenic-electron microscopy and structural modeling data. Seipin forms a decameric, cage-like structure with the lumenal domains forming a stable ring at the cage floor and transmembrane segments forming the cage sides and top. The transmembrane segments interact with adjacent monomers in two distinct, alternating conformations. These conformations result from changes in switch regions, located between the lumenal domains and the transmembrane segments, that are required for seipin function. Our data indicate a model for LD formation in which a closed seipin cage enables triacylglycerol phase separation and subsequently switches to an open conformation to allow LD growth and budding.
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| Seipin forms a flexible cage at lipid droplet formation sites.,Arlt H, Sui X, Folger B, Adams C, Chen X, Remme R, Hamprecht FA, DiMaio F, Liao M, Goodman JM, Farese RV Jr, Walther TC Nat Struct Mol Biol. 2022 Feb 24. pii: 10.1038/s41594-021-00718-y. doi:, 10.1038/s41594-021-00718-y. PMID:35210614<ref>PMID:35210614</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7rsl" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Adams, C]] | | [[Category: Adams C]] |
| [[Category: Arlt, H]] | | [[Category: Arlt H]] |
| [[Category: Chen, X]] | | [[Category: Chen X]] |
| [[Category: DiMaio, F]] | | [[Category: DiMaio F]] |
| [[Category: Folger, B]] | | [[Category: Farese Jr RV]] |
| [[Category: Goodman, J M]] | | [[Category: Folger B]] |
| [[Category: Hamprecht, F A]] | | [[Category: Goodman JM]] |
| [[Category: Jr, R V.Farese]] | | [[Category: Hamprecht FA]] |
| [[Category: Liao, M]] | | [[Category: Liao M]] |
| [[Category: Remme, R]] | | [[Category: Remme R]] |
| [[Category: Sui, X]] | | [[Category: Sui X]] |
| [[Category: Walther, T C]] | | [[Category: Walther TC]] |
| [[Category: Complex]]
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| [[Category: Endoplasmic reticulum]]
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| [[Category: Fat storage]]
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| [[Category: Lipid droplet formation]]
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| [[Category: Lipid droplet]]
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| [[Category: Membrane protein]]
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