2mwz: Difference between revisions
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==Xanthine and 8-oxoguanine in G-quadruplexes: formation of a G G X O tetrad== | ==Xanthine and 8-oxoguanine in G-quadruplexes: formation of a G G X O tetrad== | ||
<StructureSection load='2mwz' size='340' side='right'caption='[[2mwz | <StructureSection load='2mwz' size='340' side='right'caption='[[2mwz]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2mwz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MWZ FirstGlance]. <br> | <table><tr><td colspan='2'>[[2mwz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MWZ FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ZO:2-DEOXY-XANTHOSINE-5-MONOPHOSPHATE'>3ZO</scene>, <scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwz OCA], [https://pdbe.org/2mwz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwz RCSB], [https://www.ebi.ac.uk/pdbsum/2mwz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwz ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwz OCA], [https://pdbe.org/2mwz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwz RCSB], [https://www.ebi.ac.uk/pdbsum/2mwz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| Line 22: | Line 22: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cheong | [[Category: Cheong VV]] | ||
[[Category: Heddi | [[Category: Heddi B]] | ||
[[Category: Lech | [[Category: Lech CJ]] | ||
[[Category: Phan | [[Category: Phan AT]] | ||
Latest revision as of 09:11, 15 May 2024
Xanthine and 8-oxoguanine in G-quadruplexes: formation of a G G X O tetradXanthine and 8-oxoguanine in G-quadruplexes: formation of a G G X O tetrad
Structural highlights
Publication Abstract from PubMedG-quadruplexes are four-stranded structures built from stacked G-tetrads (G.G.G.G), which are planar cyclical assemblies of four guanine bases interacting through Hoogsteen hydrogen bonds. A G-quadruplex containing a single guanine analog substitution, such as 8-oxoguanine (O) or xanthine (X), would suffer from a loss of a Hoogsteen hydrogen bond within a G-tetrad and/or potential steric hindrance. We show that a proper arrangement of O and X bases can reestablish the hydrogen-bond pattern within a G.G.X.O tetrad. Rational incorporation of G.G.X.O tetrads in a (3+1) G-quadruplex demonstrated a similar folding topology and thermal stability to that of the unmodified G-quadruplex. pH titration conducted on X.O-modified G-quadruplexes indicated a protonation-deprotonation equilibrium of X with a pKa approximately 6.7. The solution structure of a G-quadruplex containing a G.G.X.O tetrad was determined, displaying the same folding topology in both the protonated and deprotonated states. A G-quadruplex containing a deprotonated X.O pair was shown to exhibit a more electronegative groove compared to that of the unmodified one. These differences are likely to manifest in the electronic properties of G-quadruplexes and may have important implications for drug targeting and DNA-protein interactions. Xanthine and 8-oxoguanine in G-quadruplexes: formation of a G.G.X.O tetrad.,Cheong VV, Heddi B, Lech CJ, Phan AT Nucleic Acids Res. 2015 Sep 22. pii: gkv826. PMID:26400177[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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