Recombinase A: Difference between revisions

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Alexander Berchansky, Michal Harel, Jaime Prilusky, Taylor Light, Joel L. Sussman

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 == Function ==
-[[Recombinase A]] (RecA), a naturally aggregating protein  involved in DNA repair, is an important asset to the genetic integrity of the ''Escherichia coli'' (''E. coli'') genome.<ref name=Shan> Shan, Q.; Cox, M. M.; Inman, R. B. DNA Strand Exchange Promoted by RecA K72R. J. Biol. Chem. 1996, 271, 5712-5724. DOI:10.1074/jbc.271.10.5712 </ref> The survival of all species rely on such DNA repair processes. RecA homologues are found in all kingdoms including archaebacteria, eubacteria, and eukaryotes.<ref name=Brendel> Brendel, V.; Brocchieri, L.; Sandler, S.J.; Clark, A.J.; Karlin, S. Evolutionary comparisons of RecA-like proteins across all major kingdoms of living organisms. J. Mol. Evol. 1997, 44, 528-541. DOI: 10.1007/PL00006177 </ref> Rad51, for example, is a RecA homologue found specifically in humans.<ref name=Baumann> Baumann, P.; Benson, F. E.; West, S. C. Human Rad51 Protein Promotes ATP-Dependent Homologous Pairing and Strand Transfer Reactions in Vitro. Cell. 1996, 87, 757-766. DOI: 10.1016/S0092-8674(00)81394-X </ref>  An over-expression of Rad51 in the nuclei of tumor cells when compared to those of normal breast tissue has been linked to sporadic, non-hereditary, breast cancers.<ref name=Maacke> Maacke, H.; Opitz, S.; Jost, K.; Hamdorf, W.; Henning, W. Krüger, S. Feller, A.C.; Lopens, A.; Diedrich, K.; Schwinger, E.; Stürzbecher, H.W. Over-expression of wild-type Rad51 correlates with histological grading of invasive ductal breast cancer. Int. J. Cancer. 2000, 88, 907-913. DOI: 10.1002/1097-0215(20001215)88:63.0.CO;2-4 </ref>  See also [[Isomerases]].
+[[Recombinase A]] (RecA), a naturally aggregating protein  involved in DNA repair, is an important asset to the genetic integrity of the ''Escherichia coli'' (''E. coli'') genome.<ref name=Shan> Shan, Q.; Cox, M. M.; Inman, R. B. DNA Strand Exchange Promoted by RecA K72R. J. Biol. Chem. 1996, 271, 5712-5724. DOI:10.1074/jbc.271.10.5712 </ref> The survival of all species rely on such DNA repair processes. RecA homologues are found in all kingdoms including archaebacteria, eubacteria, and eukaryotes.<ref name=Brendel> Brendel, V.; Brocchieri, L.; Sandler, S.J.; Clark, A.J.; Karlin, S. Evolutionary comparisons of RecA-like proteins across all major kingdoms of living organisms. J. Mol. Evol. 1997, 44, 528-541. DOI: 10.1007/PL00006177 </ref> Rad51, for example, is a RecA homologue found specifically in humans.<ref name=Baumann> Baumann, P.; Benson, F. E.; West, S. C. Human Rad51 Protein Promotes ATP-Dependent Homologous Pairing and Strand Transfer Reactions in Vitro. Cell. 1996, 87, 757-766. DOI: 10.1016/S0092-8674(00)81394-X </ref>  An over-expression of Rad51 in the nuclei of tumor cells when compared to those of normal breast tissue has been linked to sporadic, non-hereditary, breast cancers.<ref name=Maacke> Maacke, H.; Opitz, S.; Jost, K.; Hamdorf, W.; Henning, W. Krüger, S. Feller, A.C.; Lopens, A.; Diedrich, K.; Schwinger, E.; Stürzbecher, H.W. Over-expression of wild-type Rad51 correlates with histological grading of invasive ductal breast cancer. Int. J. Cancer. 2000, 88, 907-913. DOI: 10.1002/1097-0215(20001215)88:63.0.CO;2-4 </ref>  See also [[Isomerases]], [[DNA Repair]].
 == DNA Repair ==
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 <references/>
-== 3D Structures of Recombinase A ==
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-{{#tree:id=OrganizedByTopic|openlevels=0|
-* RecA
-**[[2oe2]], [[2ofo]], [[1ubc]] – ''Mycobacterium smegmatis''<br />
-**[[2zr7]] – MsRecA form II’ – MsRecA<br />
-**[[2zr0]] – MsRecA (mutant)<br />
-**[[2zrb]] - MsRecA (mutant) form II’<br />
-**[[2zrn]] - MsRecA (mutant) form IV<br />
-**[[2zrc]], [[2zrh]] - MsRecA (mutant) form IV<br />
-**[[1n03]], [[2reb]], [[3cmv]], [[4twz]] – EcRecA - ''Escherichia coli''<br />
-**[[2rec]] – EcRecA - EM<br />
-**[[1aa3]] – EcRecA C-terminal - NMR<br />
-**[[1u94]] – EcRecA form II<br />
-**[[1u98]] - EcRecA form III<br />
-**[[1u99]] - EcRecA form IV<br />
-**[[1g19]], [[4oqf]], [[4po1]], [[4po8]], [[4po9]], [[4poa]], [[4ppf]], [[4ppg]], [[4ppn]], [[4ppq]], [[4pqf]], [[4pqr]], [[4pqy]], [[4pr0]], [[4psa]], [[4psk]], [[4psv]], [[4ptl]] – MtRecA - ''Mycobacterium tuberculosis''<br />
-**[[3ifj]], [[3igd]] – MtRecA (mutant)<br />
-**[[3hr8]] – RecA – ''Thermotoga maritima''<br />
-**[[5jrj]] – RecA – ''Herbaspirillum seropedicae''<br />
-* RecA+nucleotides
-**[[2zr9]] - MsRecA (mutant) form IV+dATP<br />
-**[[2zra]] - MsRecA (mutant)+ATPgS<br />
-**[[2zrg]], [[2zrl]], [[2zrp]] - MsRecA (mutant) form II’+dATP<br />
-**[[2zrd]] - MsRecA (mutant) form IV+ADP<br />
-**[[2zre]], [[2zrj]] - MsRecA (mutant) form IV+ATPgS<br />
-**[[2zrf]], [[2zrk]], [[2zrm]] - MsRecA (mutant) form IV+dATP<br />
-**[[2zri]], [[2zro]] - MsRecA (mutant) form IV+ADP<br />
-**[[2odn]], [[2g88]], [[1ubg]] – MsRecA+dATP<br />
-**[[2odw]], [[1ubf]] - MsRecA+ATPgS<br />
-**[[2oep]], [[1ube]] - MsRecA+ADP<br />
-**[[2oes]] - MsRecA+SSB<br />
-**[[3cmt]], [[3cmu]], [[3cmw]], [[3cmx]] – EcRecA+SSDNA/DSDNA<br />
-**[[1xms]] - EcRecA+Mn+AMP-PNP<br />
-**[[1xmv]] - EcRecA+Mg+ADP<br />
-**[[1rea]] - EcRecA+ADP<br />
-**[[7jy6]], [[7jy7]], [[7jy8]], [[7jy9]] - EcRecA+ DNA + ATPgS – Cryo EM<br />
-**[[1xp8]] – RecA+ATPgS – ''Deinococcus radiodurans''<br />
-**[[1mo3]] – MtRecA+ADP<br />
-**[[1mo4]] - MtRecA+ATPgS<br />
-**[[1mo5]] - MtRecA+ATPgS+Mg<br />
-**[[1mo6]] - MtRecA+dADP+Mg<br />
-**[[1g18]] - MtRecA+ADP+AlF4<br />
-**[[5j4j]] - RecA + ADP + ATP - ''Herbaspirillum seropedicae''<br />
-}}
 [[Category:Topic Page]]