1doa: Difference between revisions

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OCA

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-[[Image:1doa.gif|left|200px]]<br />
-<applet load="1doa" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1doa, resolution 2.60&Aring;" />
-'''STRUCTURE OF THE RHO FAMILY GTP-BINDING PROTEIN CDC42 IN COMPLEX WITH THE MULTIFUNCTIONAL REGULATOR RHOGDI'''<br />
-==Overview==
+==Structure of the rho family gtp-binding protein cdc42 in complex with the multifunctional regulator rhogdi==
-The RhoGDI proteins serve as key multifunctional regulators of Rho family, GTP-binding proteins. The 2.6 A X-ray crystallographic structure of the, Cdc42/RhoGDI complex reveals two important sites of interaction between, GDI and Cdc42. First, the amino-terminal regulatory arm of the GDI binds, to the switch I and II domains of Cdc42 leading to the inhibition of both, GDP dissociation and GTP hydrolysis. Second, the geranylgeranyl moiety of, Cdc42 inserts into a hydrophobic pocket within the immunoglobulin-like, domain of the GDI molecule leading to membrane release. The structural, data demonstrate how GDIs serve as negative regulators of small, GTP-binding proteins and how the isoprenoid moiety is utilized in this, critical regulatory interaction.
+<StructureSection load='1doa' size='340' side='right'caption='[[1doa]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1doa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DOA FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMT:O-METHYLCYSTEINE'>CMT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1doa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1doa OCA], [https://pdbe.org/1doa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1doa RCSB], [https://www.ebi.ac.uk/pdbsum/1doa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1doa ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/do/1doa_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1doa ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-DOA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, GDP and GER as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DOA OCA].
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
+*[[Guanine nucleotide dissociation inhibitor|Guanine nucleotide dissociation inhibitor]]
-==Reference==
+== References ==
-Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI., Hoffman GR, Nassar N, Cerione RA, Cell. 2000 Feb 4;100(3):345-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10676816 10676816]
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Cerione, R.C.]]
+[[Category: Cerione RC]]
-[[Category: Hoffman, G.R.]]
+[[Category: Hoffman GR]]
-[[Category: Nassar, N.]]
+[[Category: Nassar N]]
-[[Category: GDP]]
-[[Category: GER]]
-[[Category: MG]]
-[[Category: cdc42]]
-[[Category: gtp-binding protein]]
-[[Category: rhogdi]]
-[[Category: x-ray]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:34:20 2007''