3d0t: Difference between revisions

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<StructureSection load='3d0t' size='340' side='right'caption='[[3d0t]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
<StructureSection load='3d0t' size='340' side='right'caption='[[3d0t]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3d0t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0T FirstGlance]. <br>
<table><tr><td colspan='2'>[[3d0t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0T FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3cqx|3cqx]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bag2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0t OCA], [https://pdbe.org/3d0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0t RCSB], [https://www.ebi.ac.uk/pdbsum/3d0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0t ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0t OCA], [https://pdbe.org/3d0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0t RCSB], [https://www.ebi.ac.uk/pdbsum/3d0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/BAG2_MOUSE BAG2_MOUSE]] Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity).  
[https://www.uniprot.org/uniprot/BAG2_MOUSE BAG2_MOUSE] Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d0t ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d0t ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cochaperones are essential for Hsp70- and Hsc70-mediated folding of proteins and include nucleotide-exchange factors (NEFs) that assist protein folding by accelerating ADP-ATP exchange on Hsp70. The cochaperone Bag2 binds misfolded Hsp70 clients and also acts as an NEF, but the molecular basis for its function is unclear. We show that, rather than being a member of the Bag domain family, Bag2 contains a new type of Hsp70 NEF domain, which we call the 'brand new bag' (BNB) domain. Free and Hsc70-bound crystal structures of Bag2-BNB show its dimeric structure, in which a flanking linker helix and loop bind to Hsc70 to promote nucleotide exchange. NMR analysis demonstrates that the client binding sites and Hsc70-interaction sites of the Bag2-BNB overlap, and that Hsc70 can displace clients from Bag2-BNB, indicating a distinct mechanism for the regulation of Hsp70-mediated protein folding by Bag2.
Structural basis of nucleotide exchange and client binding by the Hsp70 cochaperone Bag2.,Xu Z, Page RC, Gomes MM, Kohli E, Nix JC, Herr AB, Patterson C, Misra S Nat Struct Mol Biol. 2008 Dec;15(12):1309-17. Epub 2008 Nov 23. PMID:19029896<ref>PMID:19029896</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3d0t" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[BAG family proteins 3D structures|BAG family proteins 3D structures]]
*[[BAG family proteins 3D structures|BAG family proteins 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lk3 transgenic mice]]
[[Category: Mus musculus]]
[[Category: Devlin, K]]
[[Category: Devlin K]]
[[Category: Misra, S]]
[[Category: Misra S]]
[[Category: Nix, J C]]
[[Category: Nix JC]]
[[Category: Xu, Z]]
[[Category: Xu Z]]
[[Category: 4-helix bundle]]
[[Category: Chaperone]]
[[Category: Coiled coil]]

Latest revision as of 12:39, 21 February 2024

Structure of the BNB domain of the Hsp70 cochaperone Bag2Structure of the BNB domain of the Hsp70 cochaperone Bag2

Structural highlights

3d0t is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.55Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BAG2_MOUSE Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

3d0t, resolution 2.55Å

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OCA
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