2e4q: Difference between revisions
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<StructureSection load='2e4q' size='340' side='right'caption='[[2e4q]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='2e4q' size='340' side='right'caption='[[2e4q]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2e4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2e4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E4Q FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e4q OCA], [https://pdbe.org/2e4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e4q RCSB], [https://www.ebi.ac.uk/pdbsum/2e4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e4q ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e4q OCA], [https://pdbe.org/2e4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e4q RCSB], [https://www.ebi.ac.uk/pdbsum/2e4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e4q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/BPHA3_PSES1 BPHA3_PSES1] This protein seems to be a 2Fe-2S ferredoxin. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fukuda | [[Category: Pseudomonas sp]] | ||
[[Category: Ishida | [[Category: Fukuda M]] | ||
[[Category: Kimura | [[Category: Ishida T]] | ||
[[Category: Kishigami | [[Category: Kimura S]] | ||
[[Category: Senda | [[Category: Kishigami S]] | ||
[[Category: Senda | [[Category: Senda M]] | ||
[[Category: Senda T]] | |||
Latest revision as of 11:34, 25 October 2023
Crystal structure of BphA3 (reduced form)Crystal structure of BphA3 (reduced form)
Structural highlights
FunctionBPHA3_PSES1 This protein seems to be a 2Fe-2S ferredoxin. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe electron transfer system of the biphenyl dioxygenase BphA, which is derived from Acidovorax sp. (formally Pseudomonas sp.) strain KKS102, is composed of an FAD-containing NADH-ferredoxin reductase (BphA4) and a Rieske-type [2Fe-2S] ferredoxin (BphA3). Biochemical studies have suggested that the whole electron transfer process from NADH to BphA3 comprises three consecutive elementary electron-transfer reactions, in which BphA3 and BphA4 interact transiently in a redox-dependent manner. Initially, BphA4 receives two electrons from NADH. The reduced BphA4 then delivers one electron each to the [2Fe-2S] cluster of the two BphA3 molecules through redox-dependent transient interactions. The reduced BphA3 transports the electron to BphA1A2, a terminal oxygenase, to support the activation of dioxygen for biphenyl dihydroxylation. In order to elucidate the molecular mechanisms of the sequential reaction and the redox-dependent interaction between BphA3 and BphA4, we determined the crystal structures of the productive BphA3-BphA4 complex, and of free BphA3 and BphA4 in all the redox states occurring in the catalytic cycle. The crystal structures of these reaction intermediates demonstrated that each elementary electron transfer induces a series of redox-dependent conformational changes in BphA3 and BphA4, which regulate the interaction between them. In addition, the conformational changes induced by the preceding electron transfer seem to induce the next electron transfer. The interplay of electron transfer and induced conformational changes seems to be critical to the sequential electron-transfer reaction from NADH to BphA3. Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin.,Senda M, Kishigami S, Kimura S, Fukuda M, Ishida T, Senda T J Mol Biol. 2007 Oct 19;373(2):382-400. Epub 2007 Aug 19. PMID:17850818[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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