7sts: Difference between revisions
New page: '''Unreleased structure''' The entry 7sts is ON HOLD Authors: Kim, Y., Maltseva, N., Tesar, C., Jedrzejczak, R., Dugan, H., Stamper, C., Wilson, P., Joachimiak, A., Center for Structura... |
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The | ==Crystal Structure of Human Fab S24-1379 in the Complex with the N-teminal Domain of Nucleocapsid Protein from SARS CoV-2== | ||
<StructureSection load='7sts' size='340' side='right'caption='[[7sts]], [[Resolution|resolution]] 2.16Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7sts]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7STS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7STS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sts OCA], [https://pdbe.org/7sts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sts RCSB], [https://www.ebi.ac.uk/pdbsum/7sts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sts ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Coronavirus nucleocapsid protein (NP) of SARS-CoV-2 plays a central role in many functions important for virus proliferation including packaging and protecting genomic RNA. The protein shares sequence, structure, and architecture with nucleocapsid proteins from betacoronaviruses. The N-terminal domain (NP(RBD)) binds RNA and the C-terminal domain is responsible for dimerization. After infection, NP is highly expressed and triggers robust host immune response. The anti-NP antibodies are not protective and not neutralizing but can effectively detect viral proliferation soon after infection. Two structures of SARS-CoV-2 NP(RBD) were determined providing a continuous model from residue 48 to 173, including RNA binding region and key epitopes. Five structures of NP(RBD) complexes with human mAbs were isolated using an antigen-bait sorting. Complexes revealed a distinct complement-determining regions and unique sets of epitope recognition. This may assist in the early detection of pathogens and designing peptide-based vaccines. Mutations that significantly increase viral load were mapped on developed, full length NP model, likely impacting interactions with host proteins and viral RNA. | |||
Epitopes recognition of SARS-CoV-2 nucleocapsid RNA binding domain by human monoclonal antibodies.,Kim Y, Maltseva N, Tesar C, Jedrzejczak R, Endres M, Ma H, Dugan HL, Stamper CT, Chang C, Li L, Changrob S, Zheng NY, Huang M, Ramanathan A, Wilson P, Michalska K, Joachimiak A iScience. 2024 Jan 19;27(2):108976. doi: 10.1016/j.isci.2024.108976. eCollection , 2024 Feb 16. PMID:38327783<ref>PMID:38327783</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7sts" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Jedrzejczak | *[[Antibody 3D structures|Antibody 3D structures]] | ||
[[Category: | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | ||
[[Category: Maltseva | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Severe acute respiratory syndrome coronavirus 2]] | |||
[[Category: Dugan H]] | |||
[[Category: Jedrzejczak R]] | |||
[[Category: Joachimiak A]] | |||
[[Category: Kim Y]] | |||
[[Category: Maltseva N]] | |||
[[Category: Stamper C]] | |||
[[Category: Tesar C]] | |||
[[Category: Wilson P]] | |||