7qa9: Difference between revisions
New page: '''Unreleased structure''' The entry 7qa9 is ON HOLD Authors: Qiang, L., Marko, M., Chaochao, F., Jialiang, C., Yifei, Z., Kuan, L., Hongjun, L., Xuncheng, S., Zhen, X., Janez, P., Chua... |
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==10bp DNA/DNA duplex== | |||
<StructureSection load='7qa9' size='340' side='right'caption='[[7qa9]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7qa9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Octodon_degus Octodon degus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QA9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qa9 OCA], [https://pdbe.org/7qa9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qa9 RCSB], [https://www.ebi.ac.uk/pdbsum/7qa9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qa9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fluorinated nucleotides are invaluable for (19) F NMR studies of nucleic acid structure and function. Here, we synthesized 4'-SCF(3) -thymidine (T 4'-SCF3 ) and incorporated it into DNA by means of solid-phase DNA synthesis. NMR studies showed that the 4'-SCF(3) group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated by various higher order DNA structures. The three magnetically equivalent fluorine atoms in 4'-SCF(3) -DNA constitute an isolated spin system, offering high (19) F NMR sensitivity and excellent resolution of the positioning of T 4'-SCF3 within various secondary and tertiary DNA structures. The high structural adaptability and high sensitivity of T 4'-SCF3 make it a valuable (19) F NMR probe for quantitatively distinguishing diverse DNA structures with single-nucleotide resolution and for monitoring the dynamics of interactions in the minor groove of double-stranded DNA. | |||
4'-SCF(3) -Labeling Constitutes a Sensitive (19) F NMR Probe for Characterization of Interactions in the Minor Groove of DNA.,Li Q, Trajkovski M, Fan C, Chen J, Zhou Y, Lu K, Li H, Su X, Xi Z, Plavec J, Zhou C Angew Chem Int Ed Engl. 2022 Nov 21;61(47):e202201848. doi: , 10.1002/anie.202201848. Epub 2022 Oct 19. PMID:36163470<ref>PMID:36163470</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7qa9" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Octodon degus]] | ||
[[Category: | [[Category: Chen J]] | ||
[[Category: | [[Category: Fan C]] | ||
[[Category: | [[Category: Li H]] | ||
[[Category: | [[Category: Li Q]] | ||
[[Category: Lu K]] | |||
[[Category: Plavec J]] | |||
[[Category: Su X]] | |||
[[Category: Trajkovski M]] | |||
[[Category: Xi Z]] | |||
[[Category: Zhou C]] | |||
[[Category: Zhou Y]] | |||
Latest revision as of 09:16, 19 June 2024
10bp DNA/DNA duplex10bp DNA/DNA duplex
Structural highlights
Publication Abstract from PubMedFluorinated nucleotides are invaluable for (19) F NMR studies of nucleic acid structure and function. Here, we synthesized 4'-SCF(3) -thymidine (T 4'-SCF3 ) and incorporated it into DNA by means of solid-phase DNA synthesis. NMR studies showed that the 4'-SCF(3) group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated by various higher order DNA structures. The three magnetically equivalent fluorine atoms in 4'-SCF(3) -DNA constitute an isolated spin system, offering high (19) F NMR sensitivity and excellent resolution of the positioning of T 4'-SCF3 within various secondary and tertiary DNA structures. The high structural adaptability and high sensitivity of T 4'-SCF3 make it a valuable (19) F NMR probe for quantitatively distinguishing diverse DNA structures with single-nucleotide resolution and for monitoring the dynamics of interactions in the minor groove of double-stranded DNA. 4'-SCF(3) -Labeling Constitutes a Sensitive (19) F NMR Probe for Characterization of Interactions in the Minor Groove of DNA.,Li Q, Trajkovski M, Fan C, Chen J, Zhou Y, Lu K, Li H, Su X, Xi Z, Plavec J, Zhou C Angew Chem Int Ed Engl. 2022 Nov 21;61(47):e202201848. doi: , 10.1002/anie.202201848. Epub 2022 Oct 19. PMID:36163470[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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