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==THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY== | ==THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY== | ||
<StructureSection load='2afp' size='340' side='right'caption='[[2afp | <StructureSection load='2afp' size='340' side='right'caption='[[2afp]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2afp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2afp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afp OCA], [https://pdbe.org/2afp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afp RCSB], [https://www.ebi.ac.uk/pdbsum/2afp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afp ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 5 models</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afp OCA], [https://pdbe.org/2afp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afp RCSB], [https://www.ebi.ac.uk/pdbsum/2afp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/ISP2_HEMAM ISP2_HEMAM] Antifreeze proteins lower the blood freezing point. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2afp_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2afp_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| Line 34: | Line 35: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Hemitripterus americanus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Daugulis | [[Category: Daugulis AJ]] | ||
[[Category: Davies | [[Category: Davies PL]] | ||
[[Category: Gronwald | [[Category: Gronwald W]] | ||
[[Category: Lix | [[Category: Lix B]] | ||
[[Category: Loewen | [[Category: Loewen MC]] | ||
[[Category: Sonnichsen | [[Category: Sonnichsen FD]] | ||
[[Category: Sykes | [[Category: Sykes BD]] | ||
Latest revision as of 10:33, 9 October 2024
THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILYTHE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY
Structural highlights
FunctionISP2_HEMAM Antifreeze proteins lower the blood freezing point. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedA recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%. The solution structure of type II antifreeze protein reveals a new member of the lectin family.,Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:9537986[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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