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| [[Image:1fhy.gif|left|200px]]
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| <!--
| | ==PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION== |
| The line below this paragraph, containing "STRUCTURE_1fhy", creates the "Structure Box" on the page.
| | <StructureSection load='1fhy' size='340' side='right'caption='[[1fhy]], [[Resolution|resolution]] 2.20Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1fhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FHY FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
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| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PSO:4-HYDROXYMETHYL-4,5,8-TRIMETHYLPSORALEN'>PSO</scene></td></tr> |
| {{STRUCTURE_1fhy| PDB=1fhy | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhy OCA], [https://pdbe.org/1fhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fhy RCSB], [https://www.ebi.ac.uk/pdbsum/1fhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhy ProSAT]</span></td></tr> |
| | | </table> |
| '''PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION'''
| | __TOC__ |
| | | </StructureSection> |
| | | [[Category: Large Structures]] |
| ==Overview== | | [[Category: Alberti M]] |
| The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
| | [[Category: Eichman BF]] |
| | | [[Category: Hearst JE]] |
| ==About this Structure==
| | [[Category: Ho PS]] |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA]. | | [[Category: Mooers BHM]] |
| | |
| ==Reference== | |
| The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11302703 11302703]
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| [[Category: Alberti, M.]]
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| [[Category: Eichman, B F.]]
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| [[Category: Hearst, J E.]]
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| [[Category: Ho, P S.]]
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| [[Category: Mooers, B H.M.]] | |
| [[Category: 4-way junction]] | |
| [[Category: Cross-linked dna]] | |
| [[Category: Dna-drug complex]] | |
| [[Category: Holliday junction]] | |
| [[Category: Psoralen]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:20:48 2008''
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