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==== | ==Model of the outer rings of the human nuclear pore complex== | ||
< | <SX load='7peq' size='340' side='right' viewer='molstar' caption='[[7peq]], [[Resolution|resolution]] 35.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7peq]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PEQ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7peq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7peq OCA], [https://pdbe.org/7peq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7peq RCSB], [https://www.ebi.ac.uk/pdbsum/7peq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7peq ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 35Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7peq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7peq OCA], [https://pdbe.org/7peq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7peq RCSB], [https://www.ebi.ac.uk/pdbsum/7peq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7peq ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/NU133_HUMAN NU133_HUMAN] Involved in poly(A)+ RNA transport.<ref>PMID:11684705</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nuclear pore complexes (NPCs) create large conduits for cargo transport between the nucleus and cytoplasm across the nuclear envelope (NE)(1-3). These multi-megadalton structures are composed of about thirty different nucleoporins that are distributed in three main substructures (the inner, cytoplasmic and nucleoplasmic rings) around the central transport channel(4-6). Here we use cryo-electron tomography on DLD-1 cells that were prepared using cryo-focused-ion-beam milling to generate a structural model for the human NPC in its native environment. We show that-compared with previous human NPC models obtained from purified NEs-the inner ring in our model is substantially wider; the volume of the central channel is increased by 75% and the nucleoplasmic and cytoplasmic rings are reorganized. Moreover, the NPC membrane exhibits asymmetry around the inner-ring complex. Using targeted degradation of Nup96, a scaffold nucleoporin of the cytoplasmic and nucleoplasmic rings, we observe the interdependence of each ring in modulating the central channel and maintaining membrane asymmetry. Our findings highlight the inherent flexibility of the NPC and suggest that the cellular environment has a considerable influence on NPC dimensions and architecture. | |||
The cellular environment shapes the nuclear pore complex architecture.,Schuller AP, Wojtynek M, Mankus D, Tatli M, Kronenberg-Tenga R, Regmi SG, Dip PV, Lytton-Jean AKR, Brignole EJ, Dasso M, Weis K, Medalia O, Schwartz TU Nature. 2021 Oct;598(7882):667-671. doi: 10.1038/s41586-021-03985-3. Epub 2021 , Oct 13. PMID:34646014<ref>PMID:34646014</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7peq" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</ | </SX> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Dasso M]] | ||
[[Category: Kronenberg-Tenga R]] | |||
[[Category: Mankus D]] | |||
[[Category: Medalia O]] | |||
[[Category: Regmi SG]] | |||
[[Category: Schuller AP]] | |||
[[Category: Schwartz TU]] | |||
[[Category: Tatli M]] | |||
[[Category: Weis K]] | |||
[[Category: Wojtynek M]] | |||
Latest revision as of 15:32, 17 July 2024
Model of the outer rings of the human nuclear pore complexModel of the outer rings of the human nuclear pore complex
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