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| [[Image:1ffz.gif|left|200px]]
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| <!--
| | ==LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH R(CC)-DA-PUROMYCIN== |
| The line below this paragraph, containing "STRUCTURE_1ffz", creates the "Structure Box" on the page.
| | <StructureSection load='1ffz' size='340' side='right'caption='[[1ffz]], [[Resolution|resolution]] 3.20Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1ffz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FFZ FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| --> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PU:PUROMYCIN-N-AMINOPHOSPHONIC+ACID'>PU</scene></td></tr> |
| {{STRUCTURE_1ffz| PDB=1ffz | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ffz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ffz OCA], [https://pdbe.org/1ffz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ffz RCSB], [https://www.ebi.ac.uk/pdbsum/1ffz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ffz ProSAT]</span></td></tr> |
| | </table> |
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| '''LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH R(CC)-DA-PUROMYCIN'''
| | ==See Also== |
| | | *[[Large Ribosomal Subunit of Haloarcula|Large Ribosomal Subunit of Haloarcula]] |
| | | *[[Ribosome|Ribosome]] |
| ==Overview== | | __TOC__ |
| Using the atomic structures of the large ribosomal subunit from Haloarcula marismortui and its complexes with two substrate analogs, we establish that the ribosome is a ribozyme and address the catalytic properties of its all-RNA active site. Both substrate analogs are contacted exclusively by conserved ribosomal RNA (rRNA) residues from domain V of 23S rRNA; there are no protein side-chain atoms closer than about 18 angstroms to the peptide bond being synthesized. The mechanism of peptide bond synthesis appears to resemble the reverse of the acylation step in serine proteases, with the base of A2486 (A2451 in Escherichia coli) playing the same general base role as histidine-57 in chymotrypsin. The unusual pK(a) (where K(a) is the acid dissociation constant) required for A2486 to perform this function may derive in part from its hydrogen bonding to G2482 (G2447 in E. coli), which also interacts with a buried phosphate that could stabilize unusual tautomers of these two bases. The polypeptide exit tunnel is largely formed by RNA but has significant contributions from proteins L4, L22, and L39e, and its exit is encircled by proteins L19, L22, L23, L24, L29, and L31e.
| | </StructureSection> |
| | |
| ==About this Structure==
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| 1FFZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FFZ OCA].
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| ==Reference==
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| The structural basis of ribosome activity in peptide bond synthesis., Nissen P, Hansen J, Ban N, Moore PB, Steitz TA, Science. 2000 Aug 11;289(5481):920-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10937990 10937990]
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| [[Category: Haloarcula marismortui]] | | [[Category: Haloarcula marismortui]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Ban, N.]] | | [[Category: Synthetic construct]] |
| [[Category: Hansen, J.]] | | [[Category: Ban N]] |
| [[Category: Moore, P B.]] | | [[Category: Hansen J]] |
| [[Category: Nissen, P.]] | | [[Category: Moore PB]] |
| [[Category: Steitz, T A.]] | | [[Category: Nissen P]] |
| [[Category: Ribosomal rna]]
| | [[Category: Steitz TA]] |
| [[Category: Ribosome assembly]]
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| [[Category: Ribozyme]]
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| [[Category: Rna-rna complex]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:16:48 2008''
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