6d00: Difference between revisions

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<SX load='6d00' size='340' side='right' viewer='molstar' caption='[[6d00]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
<SX load='6d00' size='340' side='right' viewer='molstar' caption='[[6d00]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6d00]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_22718 Atcc 22718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D00 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6d00]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Calcarisporiella_thermophila Calcarisporiella thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D00 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6azy|6azy]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d00 OCA], [https://pdbe.org/6d00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d00 RCSB], [https://www.ebi.ac.uk/pdbsum/6d00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d00 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d00 OCA], [https://pdbe.org/6d00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d00 RCSB], [https://www.ebi.ac.uk/pdbsum/6d00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d00 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A452CSQ7_9FUNG A0A452CSQ7_9FUNG]
Hsp104 is an AAA+ protein disaggregase with powerful amyloid-remodeling activity. All nonmetazoan eukaryotes express Hsp104 while eubacteria express an Hsp104 ortholog, ClpB. However, most studies have focused on Hsp104 from Saccharomyces cerevisiae and ClpB orthologs from two eubacterial species. Thus, the natural spectrum of Hsp104/ClpB molecular architectures and protein-remodeling activities remains largely unexplored. Here, we report two structures of Hsp104 from the thermophilic fungus Calcarisporiella thermophila (CtHsp104), a 2.70A crystal structure and 4.0A cryo-electron microscopy structure. Both structures reveal left-handed, helical assemblies with all domains clearly resolved. We thus provide the highest resolution and most complete view of Hsp104 hexamers to date. We also establish that CtHsp104 antagonizes several toxic protein-misfolding events in vivo where S. cerevisiae Hsp104 is ineffective, including rescue of TDP-43, polyglutamine, and alpha-synuclein toxicity. We suggest that natural Hsp104 variation is an invaluable, untapped resource for illuminating therapeutic disaggregases for fatal neurodegenerative diseases.
 
Structure of Calcarisporiella thermophila Hsp104 Disaggregase that Antagonizes Diverse Proteotoxic Misfolding Events.,Michalska K, Zhang K, March ZM, Hatzos-Skintges C, Pintilie G, Bigelow L, Castellano LM, Miles LJ, Jackrel ME, Chuang E, Jedrzejczak R, Shorter J, Chiu W, Joachimiak A Structure. 2019 Mar 5;27(3):449-463.e7. doi: 10.1016/j.str.2018.11.001. Epub 2018, Dec 27. PMID:30595457<ref>PMID:30595457</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6d00" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Heat Shock Protein structures|Heat Shock Protein structures]]
*[[Heat Shock Protein structures|Heat Shock Protein structures]]
== References ==
<references/>
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__TOC__
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</SX>
[[Category: Atcc 22718]]
[[Category: Calcarisporiella thermophila]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pintilie, G]]
[[Category: Pintilie G]]
[[Category: Zhang, K]]
[[Category: Zhang K]]
[[Category: Adp-binding state]]
[[Category: Antagonize toxin]]
[[Category: Chaperone]]
[[Category: Spiral hexamer]]

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