2vs6: Difference between revisions
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<StructureSection load='2vs6' size='340' side='right'caption='[[2vs6]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='2vs6' size='340' side='right'caption='[[2vs6]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vs6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VS6 FirstGlance]. <br> | <table><tr><td colspan='2'>[[2vs6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichosanthes_kirilowii Trichosanthes kirilowii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VS6 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vs6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vs6 OCA], [https://pdbe.org/2vs6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vs6 RCSB], [https://www.ebi.ac.uk/pdbsum/2vs6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vs6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vs6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vs6 OCA], [https://pdbe.org/2vs6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vs6 RCSB], [https://www.ebi.ac.uk/pdbsum/2vs6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vs6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/RIPT_TRIKI RIPT_TRIKI] Inactivates eukaryotic 60S ribosomal subunits. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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==See Also== | ==See Also== | ||
*[[Ribosome inactivating protein|Ribosome inactivating protein]] | *[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Trichosanthes kirilowii]] | ||
[[Category: Au | [[Category: Au SW]] | ||
[[Category: Ma | [[Category: Ma MK]] | ||
[[Category: Mak | [[Category: Mak AN]] | ||
[[Category: Ng | [[Category: Ng A]] | ||
[[Category: Shaw | [[Category: Shaw PC]] | ||
[[Category: Too | [[Category: Too PH]] | ||
[[Category: Tung | [[Category: Tung CK]] | ||
[[Category: Wong | [[Category: Wong KB]] | ||
[[Category: Zhu | [[Category: Zhu G]] | ||
Latest revision as of 18:30, 13 December 2023
K173A, R174A, K177A-trichosanthinK173A, R174A, K177A-trichosanthin
Structural highlights
FunctionRIPT_TRIKI Inactivates eukaryotic 60S ribosomal subunits. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRibosome-inactivating proteins (RIPs) inhibit protein synthesis by enzymatically depurinating a specific adenine residue at the sarcin-ricin loop of the 28S rRNA, which thereby prevents the binding of elongation factors to the GTPase activation centre of the ribosome. Here, we present the 2.2 A crystal structure of trichosanthin (TCS) complexed to the peptide SDDDMGFGLFD, which corresponds to the conserved C-terminal elongation factor binding domain of the ribosomal P protein. The N-terminal region of this peptide interacts with Lys173, Arg174 and Lys177 in TCS, while the C-terminal region is inserted into a hydrophobic pocket. The interaction with the P protein contributes to the ribosome-inactivating activity of TCS. This 11-mer C-terminal P peptide can be docked with selected important plant and bacterial RIPs, indicating that a similar interaction may also occur with other RIPs. The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome.,Too PH, Ma MK, Mak AN, Wong YT, Tung CK, Zhu G, Au SW, Wong KB, Shaw PC Nucleic Acids Res. 2009 Feb;37(2):602-10. Epub 2008 Dec 10. PMID:19073700[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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