7f86: Difference between revisions
New page: '''Unreleased structure''' The entry 7f86 is ON HOLD Authors: Patel, S.N., Gupta, G.D., Sonani, R.R., Singh, N.K., Kumar, V., Madamwar, D. Description: Crystal structure of Phycoerythr... |
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The | ==Crystal structure of Phycoerythrin from Halomicronema Sp. R31DM== | ||
<StructureSection load='7f86' size='340' side='right'caption='[[7f86]], [[Resolution|resolution]] 2.21Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7f86]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Halomicronema_sp._R31DM Halomicronema sp. R31DM]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F86 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene>, <scene name='pdbligand=PEB:PHYCOERYTHROBILIN'>PEB</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f86 OCA], [https://pdbe.org/7f86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f86 RCSB], [https://www.ebi.ac.uk/pdbsum/7f86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f86 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A1W1IW87_9CYAN A0A1W1IW87_9CYAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phycoerythrin (PE) is green light-absorbing pigment-protein that assists in efficient light harvesting in cyanobacteria and red-algae. PE in cyanobacteria stays less studied so far as compared to that in red algae. In this study, PE from marine cyanobacteria Halomicronema sp. R31DM is purified and subjected for its structural characterisation by X-ray crystallography in order to understand its light-harvesting characteristics. The crystal structure is solved to a resolution-limit of 2.21 A with reasonable R-factors values, 0.16/0.21 (R(work)/ R(free)). PE forms hexamer of hetero-dimers made up of two peptide chains, alpha- and beta-subunits containing 2 and 3 phycoerythrobilin (PEB) chromophores covalently attached to them, respectively. Geometry of five chromophores is analysed along with their relative position within the PE hexamer. Also, their interactions with the surrounding microenvironment are analysed. The plausible energy transfer pathways in hexamer structure have been predicted based on relative position and geometry of chromophores. This structure enriches the structural information of cyanobacterial PE in order to understand its light-harvesting capacity.Communicated by Ramaswamy H. Sarma. | |||
Crystal structure analysis of phycoerythrin from marine cyanobacterium Halomicronema.,Patel SN, Sonani RR, Gupta GD, Singh NK, Kumar V, Madamwar D J Biomol Struct Dyn. 2023 Jun;41(9):3752-3761. doi: , 10.1080/07391102.2022.2055647. Epub 2022 Mar 31. PMID:35354393<ref>PMID:35354393</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7f86" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Madamwar | <references/> | ||
[[Category: Patel | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Halomicronema sp. R31DM]] | ||
[[Category: Large Structures]] | |||
[[Category: Gupta GD]] | |||
[[Category: Kumar V]] | |||
[[Category: Madamwar D]] | |||
[[Category: Patel SN]] | |||
[[Category: Singh NK]] | |||
[[Category: Sonani RR]] | |||
Latest revision as of 20:11, 29 November 2023
Crystal structure of Phycoerythrin from Halomicronema Sp. R31DMCrystal structure of Phycoerythrin from Halomicronema Sp. R31DM
Structural highlights
FunctionPublication Abstract from PubMedPhycoerythrin (PE) is green light-absorbing pigment-protein that assists in efficient light harvesting in cyanobacteria and red-algae. PE in cyanobacteria stays less studied so far as compared to that in red algae. In this study, PE from marine cyanobacteria Halomicronema sp. R31DM is purified and subjected for its structural characterisation by X-ray crystallography in order to understand its light-harvesting characteristics. The crystal structure is solved to a resolution-limit of 2.21 A with reasonable R-factors values, 0.16/0.21 (R(work)/ R(free)). PE forms hexamer of hetero-dimers made up of two peptide chains, alpha- and beta-subunits containing 2 and 3 phycoerythrobilin (PEB) chromophores covalently attached to them, respectively. Geometry of five chromophores is analysed along with their relative position within the PE hexamer. Also, their interactions with the surrounding microenvironment are analysed. The plausible energy transfer pathways in hexamer structure have been predicted based on relative position and geometry of chromophores. This structure enriches the structural information of cyanobacterial PE in order to understand its light-harvesting capacity.Communicated by Ramaswamy H. Sarma. Crystal structure analysis of phycoerythrin from marine cyanobacterium Halomicronema.,Patel SN, Sonani RR, Gupta GD, Singh NK, Kumar V, Madamwar D J Biomol Struct Dyn. 2023 Jun;41(9):3752-3761. doi: , 10.1080/07391102.2022.2055647. Epub 2022 Mar 31. PMID:35354393[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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